Development and characterization of ultra-porous silica films made by the sol-gel method. Application to biosensing

Caroline Desfours, Sylvie Calas-Etienne, R. Horváth, Marta Martin, Csilla Gergely, Frédéric Cuisinier, Pascal Etienne

Research output: Contribution to journalArticle

Abstract

The aim of this work is to demonstrate the sensing ability of reverse-symmetry waveguides to investigate adsorption of casein and build-up of poly-L-lysine mediated casein multilayers. A first part of this study is dedicated to the elaboration and characterization of ultra-porous thin films with very low refractive indices by an appropriate sol-gel method. This will form the basis of our planar optical sensors. Optical waveguide light mode spectroscopy is a real-time and sensitive method to study protein adsorption kinetics and lipid bilayers. We used it to test the obtained waveguides for in-situ monitoring of biomolecule adsorption. As a result, significant changes in the incoupling peak position were observed during the layer-by-layer adsorption. Finally, refractive index and thickness of the adsorbed layers were established.

Original languageEnglish
Pages (from-to)435-443
Number of pages9
JournalApplied Physics A: Materials Science and Processing
Volume114
Issue number2
DOIs
Publication statusPublished - Feb 2014

Fingerprint

Silicon Dioxide
Sol-gel process
Silica
gels
silicon dioxide
Adsorption
Casein
adsorption
Caseins
Refractive index
Waveguides
refractivity
waveguides
Lipid bilayers
lysine
Optical sensors
Biomolecules
Optical waveguides
optical measuring instruments
optical waveguides

ASJC Scopus subject areas

  • Materials Science(all)
  • Chemistry(all)

Cite this

Development and characterization of ultra-porous silica films made by the sol-gel method. Application to biosensing. / Desfours, Caroline; Calas-Etienne, Sylvie; Horváth, R.; Martin, Marta; Gergely, Csilla; Cuisinier, Frédéric; Etienne, Pascal.

In: Applied Physics A: Materials Science and Processing, Vol. 114, No. 2, 02.2014, p. 435-443.

Research output: Contribution to journalArticle

Desfours, Caroline ; Calas-Etienne, Sylvie ; Horváth, R. ; Martin, Marta ; Gergely, Csilla ; Cuisinier, Frédéric ; Etienne, Pascal. / Development and characterization of ultra-porous silica films made by the sol-gel method. Application to biosensing. In: Applied Physics A: Materials Science and Processing. 2014 ; Vol. 114, No. 2. pp. 435-443.
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