Development and characterization of the voriconazole loaded lipid-based nanoparticles

Petra Füredi, Zsófia Edit Pápay, Kristóf Kovács, Borbála Dalmadi Kiss, K. Ludányi, I. Antal, I. Klebovich

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The number of topical fungal infections is growing, mostly owing to immunosuppressive therapy. Several topical fungal infections, such as eye mycoses, can be treated by local administration of antimycotic drugs. One major group of the antifungal agents is triazole, such as voriconazole (VCZ), which is used as the first line treatment of aspergillosis. A disadvantage of VCZ is its low water solubility making the drug difficult to administer in a liquid preparation. The lipid-based nanoparticles (LNP) have attracted increasing attention due to their advantageous properties. Contrarily to the conventional carrier systems, LNP can improve the poor solubility of topically used drugs, such as VCZ. Therefore, LNP represents promising alternatives to traditional carrier systems. The aim of the study was to formulate VCZ loaded lipid-based nanoparticles (VCZ-LNP) by high pressure homogenization (HPH). The developed LNPs were characterized by particle size analysis, IR spectroscopy, differential scanning calorimetry, dialysis test and antifungal efficacy studies. The particle size of the optimized nanoparticles from the selected lipid base, Witepsol® W35, was 182 ± 4.1 nm after five cycles of homogenization at 600 bar. The antifungal study confirmed that the optimized VCZ-LNP inhibited the fungus reproduction.

Original languageEnglish
Pages (from-to)184-189
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume132
DOIs
Publication statusPublished - Jan 5 2017

Fingerprint

Nanoparticles
Lipids
Mycoses
Particle Size
Solubility
Pharmaceutical Preparations
Triazoles
Aspergillosis
Dialysis
Antifungal Agents
Differential Scanning Calorimetry
Immunosuppressive Agents
Fungi
Particle size analysis
Reproduction
Voriconazole
Differential scanning calorimetry
Infrared spectroscopy
Spectrum Analysis
Particle size

Keywords

  • Antifungal study
  • Dialysis test
  • Differential scanning calorimetry (DSC)
  • FTIR spectroscopy
  • High pressure homogenization
  • Particle size analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Development and characterization of the voriconazole loaded lipid-based nanoparticles. / Füredi, Petra; Pápay, Zsófia Edit; Kovács, Kristóf; Kiss, Borbála Dalmadi; Ludányi, K.; Antal, I.; Klebovich, I.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 132, 05.01.2017, p. 184-189.

Research output: Contribution to journalArticle

Füredi, Petra ; Pápay, Zsófia Edit ; Kovács, Kristóf ; Kiss, Borbála Dalmadi ; Ludányi, K. ; Antal, I. ; Klebovich, I. / Development and characterization of the voriconazole loaded lipid-based nanoparticles. In: Journal of Pharmaceutical and Biomedical Analysis. 2017 ; Vol. 132. pp. 184-189.
@article{881500eb7ba749b18e551bac321f255c,
title = "Development and characterization of the voriconazole loaded lipid-based nanoparticles",
abstract = "The number of topical fungal infections is growing, mostly owing to immunosuppressive therapy. Several topical fungal infections, such as eye mycoses, can be treated by local administration of antimycotic drugs. One major group of the antifungal agents is triazole, such as voriconazole (VCZ), which is used as the first line treatment of aspergillosis. A disadvantage of VCZ is its low water solubility making the drug difficult to administer in a liquid preparation. The lipid-based nanoparticles (LNP) have attracted increasing attention due to their advantageous properties. Contrarily to the conventional carrier systems, LNP can improve the poor solubility of topically used drugs, such as VCZ. Therefore, LNP represents promising alternatives to traditional carrier systems. The aim of the study was to formulate VCZ loaded lipid-based nanoparticles (VCZ-LNP) by high pressure homogenization (HPH). The developed LNPs were characterized by particle size analysis, IR spectroscopy, differential scanning calorimetry, dialysis test and antifungal efficacy studies. The particle size of the optimized nanoparticles from the selected lipid base, Witepsol{\circledR} W35, was 182 ± 4.1 nm after five cycles of homogenization at 600 bar. The antifungal study confirmed that the optimized VCZ-LNP inhibited the fungus reproduction.",
keywords = "Antifungal study, Dialysis test, Differential scanning calorimetry (DSC), FTIR spectroscopy, High pressure homogenization, Particle size analysis",
author = "Petra F{\"u}redi and P{\'a}pay, {Zs{\'o}fia Edit} and Krist{\'o}f Kov{\'a}cs and Kiss, {Borb{\'a}la Dalmadi} and K. Lud{\'a}nyi and I. Antal and I. Klebovich",
year = "2017",
month = "1",
day = "5",
doi = "10.1016/j.jpba.2016.09.047",
language = "English",
volume = "132",
pages = "184--189",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",

}

TY - JOUR

T1 - Development and characterization of the voriconazole loaded lipid-based nanoparticles

AU - Füredi, Petra

AU - Pápay, Zsófia Edit

AU - Kovács, Kristóf

AU - Kiss, Borbála Dalmadi

AU - Ludányi, K.

AU - Antal, I.

AU - Klebovich, I.

PY - 2017/1/5

Y1 - 2017/1/5

N2 - The number of topical fungal infections is growing, mostly owing to immunosuppressive therapy. Several topical fungal infections, such as eye mycoses, can be treated by local administration of antimycotic drugs. One major group of the antifungal agents is triazole, such as voriconazole (VCZ), which is used as the first line treatment of aspergillosis. A disadvantage of VCZ is its low water solubility making the drug difficult to administer in a liquid preparation. The lipid-based nanoparticles (LNP) have attracted increasing attention due to their advantageous properties. Contrarily to the conventional carrier systems, LNP can improve the poor solubility of topically used drugs, such as VCZ. Therefore, LNP represents promising alternatives to traditional carrier systems. The aim of the study was to formulate VCZ loaded lipid-based nanoparticles (VCZ-LNP) by high pressure homogenization (HPH). The developed LNPs were characterized by particle size analysis, IR spectroscopy, differential scanning calorimetry, dialysis test and antifungal efficacy studies. The particle size of the optimized nanoparticles from the selected lipid base, Witepsol® W35, was 182 ± 4.1 nm after five cycles of homogenization at 600 bar. The antifungal study confirmed that the optimized VCZ-LNP inhibited the fungus reproduction.

AB - The number of topical fungal infections is growing, mostly owing to immunosuppressive therapy. Several topical fungal infections, such as eye mycoses, can be treated by local administration of antimycotic drugs. One major group of the antifungal agents is triazole, such as voriconazole (VCZ), which is used as the first line treatment of aspergillosis. A disadvantage of VCZ is its low water solubility making the drug difficult to administer in a liquid preparation. The lipid-based nanoparticles (LNP) have attracted increasing attention due to their advantageous properties. Contrarily to the conventional carrier systems, LNP can improve the poor solubility of topically used drugs, such as VCZ. Therefore, LNP represents promising alternatives to traditional carrier systems. The aim of the study was to formulate VCZ loaded lipid-based nanoparticles (VCZ-LNP) by high pressure homogenization (HPH). The developed LNPs were characterized by particle size analysis, IR spectroscopy, differential scanning calorimetry, dialysis test and antifungal efficacy studies. The particle size of the optimized nanoparticles from the selected lipid base, Witepsol® W35, was 182 ± 4.1 nm after five cycles of homogenization at 600 bar. The antifungal study confirmed that the optimized VCZ-LNP inhibited the fungus reproduction.

KW - Antifungal study

KW - Dialysis test

KW - Differential scanning calorimetry (DSC)

KW - FTIR spectroscopy

KW - High pressure homogenization

KW - Particle size analysis

UR - http://www.scopus.com/inward/record.url?scp=84991730780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991730780&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2016.09.047

DO - 10.1016/j.jpba.2016.09.047

M3 - Article

C2 - 27750101

AN - SCOPUS:84991730780

VL - 132

SP - 184

EP - 189

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -