Development and characterization of a FIA system for selective assay of l-ascorbic acid in food samples

Attila Vig, Attila Igloi, N. Adányi, G. Gyémánt, C. Csutorás, Attila Kiss

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An amperometric detector and an enzymatic reaction were combined for the measurement of l-ascorbic acid. The enzyme cell (containing immobilized ascorbate oxidase) was connected to a flow injection analyzer (FIA) system with a glassy carbon electrode as an amperometric detector. During optimization and measurements two sample injectors were used, one before and one after the enzyme cell, thus eliminating the background interferences. Subtraction of the signal area given in the presence of enzyme from the one given in the absence of enzyme was applied for measuring analyte concentrations and calibration at 400 mV. Analysis capacity of system is 25 samples/hour. The relative standard deviation (RSD) was below 5% (5 times repeated, 400 μmol/L conc.), linearity up to 400 μmol/L, limit of detection (LOD) 5 μmol/L, fitting of calibration curve in 25-400 μmol/L range was R 2 = 0.99.

Original languageEnglish
Pages (from-to)947-952
Number of pages6
JournalBioprocess and Biosystems Engineering
Volume33
Issue number8
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Ascorbic acid
Ascorbic Acid
Assays
Enzymes
Food
Injections
Calibration
Ascorbate Oxidase
Detectors
Immobilized Cells
Glassy carbon
Systems Analysis
Limit of Detection
Electrodes
Carbon

Keywords

  • Amperometry
  • Ascorbic acid
  • Biosensor
  • Flow injection analysis
  • Glassy carbon

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Development and characterization of a FIA system for selective assay of l-ascorbic acid in food samples. / Vig, Attila; Igloi, Attila; Adányi, N.; Gyémánt, G.; Csutorás, C.; Kiss, Attila.

In: Bioprocess and Biosystems Engineering, Vol. 33, No. 8, 10.2010, p. 947-952.

Research output: Contribution to journalArticle

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AU - Csutorás, C.

AU - Kiss, Attila

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