We present results of a hole-burning spectral diffusion experiment On a deuterated sample of horseradish peroxidase (substituted with free-base mesoporphyrin-IX) in a trehalose/glycerol glass. Like in earlier experiments, the spectral diffusion dynamics is well-described by a diffusion picture; the most noticeable observation is a power-law dependence of the hole-broadening on time. In addition, the comparison of the present data with our earlier experiments allows for a microscopic interpretation of the influence of trehalose on protein dynamics. Here, internal water molecules in the proteins seem to be of great importance.
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films
- Materials Chemistry