Determining Progress Directions in Layer Liquid Chromatography-Dreams and Realities

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Abstract

The innovations in layer liquid chromatography has resulted in most diverse technical versions in the last decades. In these techniques, the mobile phase migrates through the stationary phase as layer by forced-flow. The development of a pressurized ultramicro (UM) chamber was the first really successful step towards a true planar-layer version of high-performance liquid chromatography (HPLC). This chamber is the basic instrument in overpressured layer chromatography (OPLC). The OPLC with on-line detection and fractionation is an ideal solution for the efficient separation and isolation of different substances, such as antimicrobials or antineoplastics. Moreover, it is very simple to couple this system on-line and off-line with different spectroscopic and spectrometric techniques and others for characterization and identification of the prospective isolated substances. The development of multisystems (e.g., multilayer, and multidetection) shows the direction of progress in OPLC. Further innovative developments are possible in CLC/RPC, and in modern layer electrochromatographic techniques (e.g. pressurized chambers, hyphenations).The development of BioArena means an exploitation of the unique advantages of a planar-layer system for detection, isolation, and identification of new antimicrobials, antineoplastics, and others, as well as for studying biochemical reactions. (In fact, the adsorbent bed in the column arrangement is not suitable for biological detection etc.) Our more recent investigations demonstrate clearly a unique function and role of endogenous formaldehyde (HCHO) and its one reaction product, endogenous ozone (O3) in the antiproliferative (e.g., antimicrobial or antineoplastic) effect. These results can be extended for in vivo conditions (e.g., greenhouse experiments) as well.

Original languageEnglish
Title of host publicationForced-Flow Layer Chromatography
PublisherElsevier Inc.
Pages487-501
Number of pages15
ISBN (Print)9780124201613
DOIs
Publication statusPublished - Jan 1 2016

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Keywords

  • BioArena
  • Centrifugal force
  • Direct bioautography
  • Electroosmosis
  • Forced flow
  • Layer chromatography
  • Overpressured layer chromatography
  • Planar electrochromatography
  • Pressurized UM chamber
  • Shear-driven chromatography
  • UM chamber

ASJC Scopus subject areas

  • Chemistry(all)

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