Determination of Visnagin in Ammi visnaga Hairy Root Cultures Using Solid-Phase Extraction and High-Performance Liquid Chromatography

László Kursinszki, Jekaterina Troilina, Éva Szõke

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A high-performance liquid chromatographic method was developed for separation of the furochromone fraction and for determination of visnagin in Ammi visnaga hairy root cultures. Lyophilized samples were extracted with chloroform:methanol (1:1, v/v) and purified on solid-phase extraction cartridges. HPLC analyses were performed on a Eurospher 100-C8 Knauer column and the mobile phase was 29:28:526:417 (v/v/v/v) acetonitrile:tetrahydrofuran:30 mM citric acid (pH 3.0):methanol. Quercetin was used as internal standard. Peaks were identified by addition of authentic standards and/or by diode-array detection.

Original languageEnglish
Pages (from-to)392-398
Number of pages7
JournalMicrochemical Journal
Volume59
Issue number3
DOIs
Publication statusPublished - Jul 1998

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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