Determination of UTP and ATP pool sizes in human tonsillar lymphocytes by using Escherichia coli RNA polymerase

M. Sasvári, Márta Vitéz, M. Staub, F. Antoni

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The present paper describes a rapid, specific and sensitive method for quantitating ribonucleoside triphosphates (ATP and UTP) in cell extracts. The principle of the method is based on the synthesis of a ribonucleotide polymer in the presence of UTP, ATP and poly(dA-dT) as template. A method for calculation is also described, making the determination of UTP and ATP pool sizes in the cells possible under the same experimental conditions. The calculation takes into account the isotope dilution effect caused by the intracellular ATP. Our experiments show that the neutralized perchloric acid soluble fraction of human tonsillar lymphocytes contains no inhibitors for the RNA polymerase test. According to our results, this cell extract contains 80 pmol of UTP and 340 pmol of ATP per μg RNA.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume395
Issue number3
DOIs
Publication statusPublished - Jul 7 1975

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Uridine Triphosphate
DNA-Directed RNA Polymerases
Adenosine Triphosphate
Lymphocytes
Escherichia coli
Cell Extracts
Poly dA-dT
Ribonucleosides
Ribonucleotides
Cell Size
Isotopes
Polymers
RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Determination of UTP and ATP pool sizes in human tonsillar lymphocytes by using Escherichia coli RNA polymerase. / Sasvári, M.; Vitéz, Márta; Staub, M.; Antoni, F.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 395, No. 3, 07.07.1975, p. 221-228.

Research output: Contribution to journalArticle

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