Determination of two metabolites of albendazole, albendazole-sulfoxide and albendazole-sulfone in cow's milk using an HPLC method - A systematic approach to optimise extraction conditions -

Zs Romvári, J. Fekete, S. Kemény, G. Pokol, I. Gebefügi, A. Kettrup

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Reversed phase HPLC methods for the determination of two metabolites of albendazole in cow's milk are described. Albendazole is an anthelmintic agent used both in veterinary and human practice. Its main metabolites are albendazole-sulfoxide and albendazole-sulfone. The separation of the two metabolites was performed on a μ-Bondapak C-18 column using an acetonitrile/methanol/phosphate buffer as mobile phase, with detection at 290 nm. For the sample preparation of the milk samples we used solid phase extraction. A new sample treatment optimisation procedure is also presented for the sample preparation method. Since sample preparation is a critical step in the analysis of residues in biological samples, the second part of our publication describes an optimisation using Taguchi's methodology that increased recovery, accuracy and reliability. After optimisation of the sample preparation, the limit of detection was 10 ng mL-1 milk and the recovery was 70-80 % in the concentration range of 30-1000 ng mL-1 milk for both metabolites.

Original languageEnglish
Pages (from-to)777-784
Number of pages8
JournalChromatographia
Volume48
Issue number11-12
DOIs
Publication statusPublished - 1998

Fingerprint

Albendazole
Metabolites
Milk
High Pressure Liquid Chromatography
Recovery
Anthelmintics
Solid Phase Extraction
Methanol
Limit of Detection
Buffers
Phosphates
albendazole sulfone
albendazole sulfoxide

Keywords

  • Albendazole metabolites
  • Column liquid chromatography
  • Milk samples
  • Sample treatment optimisation
  • Solid phase extraction
  • Taguchi method

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

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title = "Determination of two metabolites of albendazole, albendazole-sulfoxide and albendazole-sulfone in cow's milk using an HPLC method - A systematic approach to optimise extraction conditions -",
abstract = "Reversed phase HPLC methods for the determination of two metabolites of albendazole in cow's milk are described. Albendazole is an anthelmintic agent used both in veterinary and human practice. Its main metabolites are albendazole-sulfoxide and albendazole-sulfone. The separation of the two metabolites was performed on a μ-Bondapak C-18 column using an acetonitrile/methanol/phosphate buffer as mobile phase, with detection at 290 nm. For the sample preparation of the milk samples we used solid phase extraction. A new sample treatment optimisation procedure is also presented for the sample preparation method. Since sample preparation is a critical step in the analysis of residues in biological samples, the second part of our publication describes an optimisation using Taguchi's methodology that increased recovery, accuracy and reliability. After optimisation of the sample preparation, the limit of detection was 10 ng mL-1 milk and the recovery was 70-80 {\%} in the concentration range of 30-1000 ng mL-1 milk for both metabolites.",
keywords = "Albendazole metabolites, Column liquid chromatography, Milk samples, Sample treatment optimisation, Solid phase extraction, Taguchi method",
author = "Zs Romv{\'a}ri and J. Fekete and S. Kem{\'e}ny and G. Pokol and I. Gebef{\"u}gi and A. Kettrup",
year = "1998",
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volume = "48",
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AU - Romvári, Zs

AU - Fekete, J.

AU - Kemény, S.

AU - Pokol, G.

AU - Gebefügi, I.

AU - Kettrup, A.

PY - 1998

Y1 - 1998

N2 - Reversed phase HPLC methods for the determination of two metabolites of albendazole in cow's milk are described. Albendazole is an anthelmintic agent used both in veterinary and human practice. Its main metabolites are albendazole-sulfoxide and albendazole-sulfone. The separation of the two metabolites was performed on a μ-Bondapak C-18 column using an acetonitrile/methanol/phosphate buffer as mobile phase, with detection at 290 nm. For the sample preparation of the milk samples we used solid phase extraction. A new sample treatment optimisation procedure is also presented for the sample preparation method. Since sample preparation is a critical step in the analysis of residues in biological samples, the second part of our publication describes an optimisation using Taguchi's methodology that increased recovery, accuracy and reliability. After optimisation of the sample preparation, the limit of detection was 10 ng mL-1 milk and the recovery was 70-80 % in the concentration range of 30-1000 ng mL-1 milk for both metabolites.

AB - Reversed phase HPLC methods for the determination of two metabolites of albendazole in cow's milk are described. Albendazole is an anthelmintic agent used both in veterinary and human practice. Its main metabolites are albendazole-sulfoxide and albendazole-sulfone. The separation of the two metabolites was performed on a μ-Bondapak C-18 column using an acetonitrile/methanol/phosphate buffer as mobile phase, with detection at 290 nm. For the sample preparation of the milk samples we used solid phase extraction. A new sample treatment optimisation procedure is also presented for the sample preparation method. Since sample preparation is a critical step in the analysis of residues in biological samples, the second part of our publication describes an optimisation using Taguchi's methodology that increased recovery, accuracy and reliability. After optimisation of the sample preparation, the limit of detection was 10 ng mL-1 milk and the recovery was 70-80 % in the concentration range of 30-1000 ng mL-1 milk for both metabolites.

KW - Albendazole metabolites

KW - Column liquid chromatography

KW - Milk samples

KW - Sample treatment optimisation

KW - Solid phase extraction

KW - Taguchi method

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