Determination of the125J-insulin-plasma complex by gel filtration

Janos Földes, Edit Piroska, Gyula Tamás

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The capacity of plasma proteins to bind insulin labelled with125J has been studied by means of the gel-filtration method. Fractionation with Sephadex G-100 showed that only 10 % of the labelled insulin was bound by the plasma proteins of healthy subjects, pregnant females and untreated diabetes mellitus patients. A much higher percentage of insulin was bound by plasma proteins in patients who had previously been treated with beef insulin, while the degree of binding became extreme in insulin-resistant diabetics. Experiments made with Sephadex G-200 showed that, following short-term insulin therapy, the insulin-protein complex migrated with the globulins 19 S. After prolonged insulin therapy and in insulin-resistant cases most of the protein-bound labelled insulin was eluted with the globulins 7 S. The phenomenon, discussed in detail, is attributed to the action of beef insulin antibodies.

Original languageEnglish
Pages (from-to)347-363
Number of pages17
JournalActa Diabetologica Latina
Volume5
Issue number3
DOIs
Publication statusPublished - Jul 1 1968

Keywords

  • Diabetes mellitus
  • Gel-filtration
  • Insulin
  • J-insulin-plasma complex

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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