The capacity of plasma proteins to bind insulin labelled with125J has been studied by means of the gel-filtration method. Fractionation with Sephadex G-100 showed that only 10 % of the labelled insulin was bound by the plasma proteins of healthy subjects, pregnant females and untreated diabetes mellitus patients. A much higher percentage of insulin was bound by plasma proteins in patients who had previously been treated with beef insulin, while the degree of binding became extreme in insulin-resistant diabetics. Experiments made with Sephadex G-200 showed that, following short-term insulin therapy, the insulin-protein complex migrated with the globulins 19 S. After prolonged insulin therapy and in insulin-resistant cases most of the protein-bound labelled insulin was eluted with the globulins 7 S. The phenomenon, discussed in detail, is attributed to the action of beef insulin antibodies.
- Diabetes mellitus
- J-insulin-plasma complex
ASJC Scopus subject areas
- Internal Medicine
- Endocrinology, Diabetes and Metabolism