Determination of taxol in Taxus species grown in Hungary by high-performance liquid chromatography-diode array detection. Effect of vegetative period

Veronika Németh-Kiss, E. Forgács, T. Cserháti, Gábor Schmidt

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The influence of a vegetative period on the taxol content in the needles of Taxus brevifolia grown in Hungary was determined using porous graphitized carbon column and a HPLC-diode array detection system. The relative standard deviation of the retention time of taxol peak was 1.24%, the peak symmetry 1.06-1.07 indicating the reliability of the HPLC systems. It was found that the accuracy of the peak purity test can be enhanced by carrying out the test at various wavelengths. It was established that the taxol content is considerably higher in the winter months and taxol is purer in the same period. Taxol content also depends on the production site and taxol extracted from the foliage contains more taxoteres than taxol extracted from bark.

Original languageEnglish
Pages (from-to)253-256
Number of pages4
JournalJournal of Chromatography A
Volume750
Issue number1-2
DOIs
Publication statusPublished - Oct 25 1996

Fingerprint

Taxus
Hungary
High performance liquid chromatography
Paclitaxel
Diodes
High Pressure Liquid Chromatography
docetaxel
Needles
Carbon
Wavelength

Keywords

  • Taxol
  • Taxus baccata

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of taxol in Taxus species grown in Hungary by high-performance liquid chromatography-diode array detection. Effect of vegetative period. / Németh-Kiss, Veronika; Forgács, E.; Cserháti, T.; Schmidt, Gábor.

In: Journal of Chromatography A, Vol. 750, No. 1-2, 25.10.1996, p. 253-256.

Research output: Contribution to journalArticle

@article{adbe1c6e6a544f569f172f555c13171f,
title = "Determination of taxol in Taxus species grown in Hungary by high-performance liquid chromatography-diode array detection. Effect of vegetative period",
abstract = "The influence of a vegetative period on the taxol content in the needles of Taxus brevifolia grown in Hungary was determined using porous graphitized carbon column and a HPLC-diode array detection system. The relative standard deviation of the retention time of taxol peak was 1.24{\%}, the peak symmetry 1.06-1.07 indicating the reliability of the HPLC systems. It was found that the accuracy of the peak purity test can be enhanced by carrying out the test at various wavelengths. It was established that the taxol content is considerably higher in the winter months and taxol is purer in the same period. Taxol content also depends on the production site and taxol extracted from the foliage contains more taxoteres than taxol extracted from bark.",
keywords = "Taxol, Taxus baccata",
author = "Veronika N{\'e}meth-Kiss and E. Forg{\'a}cs and T. Cserh{\'a}ti and G{\'a}bor Schmidt",
year = "1996",
month = "10",
day = "25",
doi = "10.1016/0021-9673(96)00541-9",
language = "English",
volume = "750",
pages = "253--256",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Determination of taxol in Taxus species grown in Hungary by high-performance liquid chromatography-diode array detection. Effect of vegetative period

AU - Németh-Kiss, Veronika

AU - Forgács, E.

AU - Cserháti, T.

AU - Schmidt, Gábor

PY - 1996/10/25

Y1 - 1996/10/25

N2 - The influence of a vegetative period on the taxol content in the needles of Taxus brevifolia grown in Hungary was determined using porous graphitized carbon column and a HPLC-diode array detection system. The relative standard deviation of the retention time of taxol peak was 1.24%, the peak symmetry 1.06-1.07 indicating the reliability of the HPLC systems. It was found that the accuracy of the peak purity test can be enhanced by carrying out the test at various wavelengths. It was established that the taxol content is considerably higher in the winter months and taxol is purer in the same period. Taxol content also depends on the production site and taxol extracted from the foliage contains more taxoteres than taxol extracted from bark.

AB - The influence of a vegetative period on the taxol content in the needles of Taxus brevifolia grown in Hungary was determined using porous graphitized carbon column and a HPLC-diode array detection system. The relative standard deviation of the retention time of taxol peak was 1.24%, the peak symmetry 1.06-1.07 indicating the reliability of the HPLC systems. It was found that the accuracy of the peak purity test can be enhanced by carrying out the test at various wavelengths. It was established that the taxol content is considerably higher in the winter months and taxol is purer in the same period. Taxol content also depends on the production site and taxol extracted from the foliage contains more taxoteres than taxol extracted from bark.

KW - Taxol

KW - Taxus baccata

UR - http://www.scopus.com/inward/record.url?scp=0030601769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030601769&partnerID=8YFLogxK

U2 - 10.1016/0021-9673(96)00541-9

DO - 10.1016/0021-9673(96)00541-9

M3 - Article

AN - SCOPUS:0030601769

VL - 750

SP - 253

EP - 256

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1-2

ER -