Determination of sorbitol in the presence of high amount of mannitol from biological samples

Bálint Tomasskovics, V. Horváth, József Balla, Ferenc Örsi, A. Szarka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Preparation of intact mitochondria requires the use of an osmoticum in high concentration that preserves the mitochondrial structure and prevents physical swelling and rupture of membranes. For this purpose mannitol is widely accepted as a component of the homogenization medium. The formation of sorbitol in plant mitochondria can be an important element of plant response to salinity and drought. The large difference in the concentration of possibly formed sorbitol and mannitol of the homogenisation medium makes the determination of sorbitol difficult. Gas-chromatography with flame ionization detection (GC-FID), gas-chromatography coupled to quadrupole mass spectrometry (GC-QPMS) and liquid chromatography/ tandem mass spectrometry (LC/MS-MS) using hydrophilic interaction chromatography (HILIC) were tested to quantify this highly polar sugar alcohol: Although all three methods offered satisfactory results, the HPLC/MS-MS technique proved to be the best.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalPeriodica Polytechnica: Chemical Engineering
Volume58
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Mitochondria
Sorbitol
Mannitol
Gas chromatography
Mass spectrometry
Drought
Liquid chromatography
Chromatography
Ionization
Sugar Alcohols
Swelling
Membranes

Keywords

  • Liquid chromatography/tandem mass
  • Plant mitochondria
  • Sorbitol determination

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Determination of sorbitol in the presence of high amount of mannitol from biological samples. / Tomasskovics, Bálint; Horváth, V.; Balla, József; Örsi, Ferenc; Szarka, A.

In: Periodica Polytechnica: Chemical Engineering, Vol. 58, No. 1, 2014, p. 1-6.

Research output: Contribution to journalArticle

@article{3d9a16ed643e460e9c3d908fa953be80,
title = "Determination of sorbitol in the presence of high amount of mannitol from biological samples",
abstract = "Preparation of intact mitochondria requires the use of an osmoticum in high concentration that preserves the mitochondrial structure and prevents physical swelling and rupture of membranes. For this purpose mannitol is widely accepted as a component of the homogenization medium. The formation of sorbitol in plant mitochondria can be an important element of plant response to salinity and drought. The large difference in the concentration of possibly formed sorbitol and mannitol of the homogenisation medium makes the determination of sorbitol difficult. Gas-chromatography with flame ionization detection (GC-FID), gas-chromatography coupled to quadrupole mass spectrometry (GC-QPMS) and liquid chromatography/ tandem mass spectrometry (LC/MS-MS) using hydrophilic interaction chromatography (HILIC) were tested to quantify this highly polar sugar alcohol: Although all three methods offered satisfactory results, the HPLC/MS-MS technique proved to be the best.",
keywords = "Liquid chromatography/tandem mass, Plant mitochondria, Sorbitol determination",
author = "B{\'a}lint Tomasskovics and V. Horv{\'a}th and J{\'o}zsef Balla and Ferenc {\"O}rsi and A. Szarka",
year = "2014",
doi = "10.3311/PPch.7048",
language = "English",
volume = "58",
pages = "1--6",
journal = "Periodica Polytechnica: Chemical Engineering",
issn = "0324-5853",
publisher = "Budapest University of Technology and Economics",
number = "1",

}

TY - JOUR

T1 - Determination of sorbitol in the presence of high amount of mannitol from biological samples

AU - Tomasskovics, Bálint

AU - Horváth, V.

AU - Balla, József

AU - Örsi, Ferenc

AU - Szarka, A.

PY - 2014

Y1 - 2014

N2 - Preparation of intact mitochondria requires the use of an osmoticum in high concentration that preserves the mitochondrial structure and prevents physical swelling and rupture of membranes. For this purpose mannitol is widely accepted as a component of the homogenization medium. The formation of sorbitol in plant mitochondria can be an important element of plant response to salinity and drought. The large difference in the concentration of possibly formed sorbitol and mannitol of the homogenisation medium makes the determination of sorbitol difficult. Gas-chromatography with flame ionization detection (GC-FID), gas-chromatography coupled to quadrupole mass spectrometry (GC-QPMS) and liquid chromatography/ tandem mass spectrometry (LC/MS-MS) using hydrophilic interaction chromatography (HILIC) were tested to quantify this highly polar sugar alcohol: Although all three methods offered satisfactory results, the HPLC/MS-MS technique proved to be the best.

AB - Preparation of intact mitochondria requires the use of an osmoticum in high concentration that preserves the mitochondrial structure and prevents physical swelling and rupture of membranes. For this purpose mannitol is widely accepted as a component of the homogenization medium. The formation of sorbitol in plant mitochondria can be an important element of plant response to salinity and drought. The large difference in the concentration of possibly formed sorbitol and mannitol of the homogenisation medium makes the determination of sorbitol difficult. Gas-chromatography with flame ionization detection (GC-FID), gas-chromatography coupled to quadrupole mass spectrometry (GC-QPMS) and liquid chromatography/ tandem mass spectrometry (LC/MS-MS) using hydrophilic interaction chromatography (HILIC) were tested to quantify this highly polar sugar alcohol: Although all three methods offered satisfactory results, the HPLC/MS-MS technique proved to be the best.

KW - Liquid chromatography/tandem mass

KW - Plant mitochondria

KW - Sorbitol determination

UR - http://www.scopus.com/inward/record.url?scp=84890932566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890932566&partnerID=8YFLogxK

U2 - 10.3311/PPch.7048

DO - 10.3311/PPch.7048

M3 - Article

AN - SCOPUS:84890932566

VL - 58

SP - 1

EP - 6

JO - Periodica Polytechnica: Chemical Engineering

JF - Periodica Polytechnica: Chemical Engineering

SN - 0324-5853

IS - 1

ER -