Determination of serotonin released from coffee wax by liquid chromatography

M. Kele, R. Ohmacht

Research output: Contribution to journalArticle

19 Citations (Scopus)


A simple hydrolysis and extraction method was developed for the release of serotonin (5-hydroxytryptamine) from a coffee wax sample obtained from decaffeination of coffee beans. The recoverable amount of serotonin was determined by reversed-phase high-performance liquid chromatography with gradient elution and UV detection, using the standard addition method. Different type of basic deactivated chromatographic columns were used for the separation.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - Apr 12 1996


  • Coffee
  • Phenol
  • Pyridine
  • Serotonin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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