Determination of serotonin released from coffee wax by liquid chromatography

M. Kele, R. Ohmacht

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A simple hydrolysis and extraction method was developed for the release of serotonin (5-hydroxytryptamine) from a coffee wax sample obtained from decaffeination of coffee beans. The recoverable amount of serotonin was determined by reversed-phase high-performance liquid chromatography with gradient elution and UV detection, using the standard addition method. Different type of basic deactivated chromatographic columns were used for the separation.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalJournal of Chromatography A
Volume730
Issue number1-2
DOIs
Publication statusPublished - Apr 12 1996

Fingerprint

Coffee
Waxes
Liquid chromatography
Liquid Chromatography
Serotonin
High performance liquid chromatography
Reverse-Phase Chromatography
Hydrolysis
High Pressure Liquid Chromatography

Keywords

  • Coffee
  • Phenol
  • Pyridine
  • Serotonin

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of serotonin released from coffee wax by liquid chromatography. / Kele, M.; Ohmacht, R.

In: Journal of Chromatography A, Vol. 730, No. 1-2, 12.04.1996, p. 59-62.

Research output: Contribution to journalArticle

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