Determination of selenocysteine and selenomethionine in edible animal tissues by 2D size-exclusion reversed-phase HPLC-ICP MS following carbamidomethylation and proteolytic extraction

Katarzyna Bierla, M. Dernovics, Véronique Vacchina, Joanna Szpunar, Gérard Bertin, Ryszard Lobinski

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

A method was developed for the simultaneous determination of selenomethionine (SeMet) and selenocysteine (SeCys) in meat (chicken and lamb muscles) and different offal tissues (heart, liver, kidney). The analytical procedure was based on the protein extraction with urea under reducing conditions (dithiothreitol), derivatization of SeCys and SeMet by carbamidomethylation with iodoacetamide (IAM) followed by quantitative proteolysis. The mixture of the derivatized Se-amino acids was purified by size-exclusion liquid chromatography (LC) and analysed by ion-paring reversed-phase HPLC-inductively coupled plasma mass spectroscopy (ICP MS). The quantification of SeCys and SeMet was carried out by the method of standard additions. 77SeMet was used to control the SeMet derivatization efficiency and recovery. The method was validated by the determination of the Se mass balance. The Se-amino acids accounted for 91∈±∈8% of the total selenium (mean of 95 samples of seven tissues analysed over a period of 18 months). The method was applied to the discrimination of the contribution of selenoproteins (containing SeCys) and other Se-containing proteins (containing SeMet) in tissues of animals during supplementation studies (dose-effect and tolerance).

Original languageEnglish
Pages (from-to)1789-1798
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume390
Issue number7
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Selenocysteine
Selenomethionine
Inductively coupled plasma
Mass Spectrometry
Animals
High Pressure Liquid Chromatography
Spectroscopy
Tissue
Selenoproteins
Proteolysis
Iodoacetamide
Amino Acids
Size exclusion chromatography
Meats
Dithiothreitol
Liquid chromatography
Selenium
Liquid Chromatography
Liver
Meat

Keywords

  • Animal tissues
  • HPLC-ICP MS
  • Selenocysteine
  • Selenomethionine
  • Supplementation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Determination of selenocysteine and selenomethionine in edible animal tissues by 2D size-exclusion reversed-phase HPLC-ICP MS following carbamidomethylation and proteolytic extraction. / Bierla, Katarzyna; Dernovics, M.; Vacchina, Véronique; Szpunar, Joanna; Bertin, Gérard; Lobinski, Ryszard.

In: Analytical and Bioanalytical Chemistry, Vol. 390, No. 7, 04.2008, p. 1789-1798.

Research output: Contribution to journalArticle

@article{7e3edb7529114c2c94c4f9933e9724ec,
title = "Determination of selenocysteine and selenomethionine in edible animal tissues by 2D size-exclusion reversed-phase HPLC-ICP MS following carbamidomethylation and proteolytic extraction",
abstract = "A method was developed for the simultaneous determination of selenomethionine (SeMet) and selenocysteine (SeCys) in meat (chicken and lamb muscles) and different offal tissues (heart, liver, kidney). The analytical procedure was based on the protein extraction with urea under reducing conditions (dithiothreitol), derivatization of SeCys and SeMet by carbamidomethylation with iodoacetamide (IAM) followed by quantitative proteolysis. The mixture of the derivatized Se-amino acids was purified by size-exclusion liquid chromatography (LC) and analysed by ion-paring reversed-phase HPLC-inductively coupled plasma mass spectroscopy (ICP MS). The quantification of SeCys and SeMet was carried out by the method of standard additions. 77SeMet was used to control the SeMet derivatization efficiency and recovery. The method was validated by the determination of the Se mass balance. The Se-amino acids accounted for 91∈±∈8{\%} of the total selenium (mean of 95 samples of seven tissues analysed over a period of 18 months). The method was applied to the discrimination of the contribution of selenoproteins (containing SeCys) and other Se-containing proteins (containing SeMet) in tissues of animals during supplementation studies (dose-effect and tolerance).",
keywords = "Animal tissues, HPLC-ICP MS, Selenocysteine, Selenomethionine, Supplementation",
author = "Katarzyna Bierla and M. Dernovics and V{\'e}ronique Vacchina and Joanna Szpunar and G{\'e}rard Bertin and Ryszard Lobinski",
year = "2008",
month = "4",
doi = "10.1007/s00216-008-1883-5",
language = "English",
volume = "390",
pages = "1789--1798",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "1618-2642",
publisher = "Springer Verlag",
number = "7",

}

TY - JOUR

T1 - Determination of selenocysteine and selenomethionine in edible animal tissues by 2D size-exclusion reversed-phase HPLC-ICP MS following carbamidomethylation and proteolytic extraction

AU - Bierla, Katarzyna

AU - Dernovics, M.

AU - Vacchina, Véronique

AU - Szpunar, Joanna

AU - Bertin, Gérard

AU - Lobinski, Ryszard

PY - 2008/4

Y1 - 2008/4

N2 - A method was developed for the simultaneous determination of selenomethionine (SeMet) and selenocysteine (SeCys) in meat (chicken and lamb muscles) and different offal tissues (heart, liver, kidney). The analytical procedure was based on the protein extraction with urea under reducing conditions (dithiothreitol), derivatization of SeCys and SeMet by carbamidomethylation with iodoacetamide (IAM) followed by quantitative proteolysis. The mixture of the derivatized Se-amino acids was purified by size-exclusion liquid chromatography (LC) and analysed by ion-paring reversed-phase HPLC-inductively coupled plasma mass spectroscopy (ICP MS). The quantification of SeCys and SeMet was carried out by the method of standard additions. 77SeMet was used to control the SeMet derivatization efficiency and recovery. The method was validated by the determination of the Se mass balance. The Se-amino acids accounted for 91∈±∈8% of the total selenium (mean of 95 samples of seven tissues analysed over a period of 18 months). The method was applied to the discrimination of the contribution of selenoproteins (containing SeCys) and other Se-containing proteins (containing SeMet) in tissues of animals during supplementation studies (dose-effect and tolerance).

AB - A method was developed for the simultaneous determination of selenomethionine (SeMet) and selenocysteine (SeCys) in meat (chicken and lamb muscles) and different offal tissues (heart, liver, kidney). The analytical procedure was based on the protein extraction with urea under reducing conditions (dithiothreitol), derivatization of SeCys and SeMet by carbamidomethylation with iodoacetamide (IAM) followed by quantitative proteolysis. The mixture of the derivatized Se-amino acids was purified by size-exclusion liquid chromatography (LC) and analysed by ion-paring reversed-phase HPLC-inductively coupled plasma mass spectroscopy (ICP MS). The quantification of SeCys and SeMet was carried out by the method of standard additions. 77SeMet was used to control the SeMet derivatization efficiency and recovery. The method was validated by the determination of the Se mass balance. The Se-amino acids accounted for 91∈±∈8% of the total selenium (mean of 95 samples of seven tissues analysed over a period of 18 months). The method was applied to the discrimination of the contribution of selenoproteins (containing SeCys) and other Se-containing proteins (containing SeMet) in tissues of animals during supplementation studies (dose-effect and tolerance).

KW - Animal tissues

KW - HPLC-ICP MS

KW - Selenocysteine

KW - Selenomethionine

KW - Supplementation

UR - http://www.scopus.com/inward/record.url?scp=41149124816&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41149124816&partnerID=8YFLogxK

U2 - 10.1007/s00216-008-1883-5

DO - 10.1007/s00216-008-1883-5

M3 - Article

C2 - 18283440

AN - SCOPUS:41149124816

VL - 390

SP - 1789

EP - 1798

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 1618-2642

IS - 7

ER -