Determination of selenoamino acids by high-performance liquid chromatography-hydraulic high pressure nebulization-atomic fluorescence spectrometry

Erzsebet Puskel, Zoltan Mester, P. Fodor

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

For the analysis of the most important selenoamino acids [selenomethionine (SeMet), selenoethionine (SeEt) and selenocystine (SeCys)] a HPLC-hydraulic high pressure nebulization (HHPN)-AFS system was assembled. The separation was accomplished on a C18 column with the help of trifluoroacetic acid (TFA) and methanol in water as eluent. The effects of methanol and TFA concentrations on the retention time were investigated. The argon and hydrogen gas flow rate on the AFS signal intensity was optimized. The detection limit and the retention time for SeCys, SeMet and SeEt were 50, 42 and 71 ng ml-1 and 2.1, 3.9 and 6.9 min, respectively.

Original languageEnglish
Pages (from-to)973-976
Number of pages4
JournalJournal of Analytical Atomic Spectrometry
Volume14
Issue number6
DOIs
Publication statusPublished - 1999

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Selenomethionine
Trifluoroacetic Acid
High performance liquid chromatography
Spectrometry
Methanol
Fluorescence
Hydraulics
Acids
Argon
Flow of gases
Hydrogen
Flow rate
Water
selenocystine

ASJC Scopus subject areas

  • Spectroscopy

Cite this

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abstract = "For the analysis of the most important selenoamino acids [selenomethionine (SeMet), selenoethionine (SeEt) and selenocystine (SeCys)] a HPLC-hydraulic high pressure nebulization (HHPN)-AFS system was assembled. The separation was accomplished on a C18 column with the help of trifluoroacetic acid (TFA) and methanol in water as eluent. The effects of methanol and TFA concentrations on the retention time were investigated. The argon and hydrogen gas flow rate on the AFS signal intensity was optimized. The detection limit and the retention time for SeCys, SeMet and SeEt were 50, 42 and 71 ng ml-1 and 2.1, 3.9 and 6.9 min, respectively.",
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AU - Fodor, P.

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AB - For the analysis of the most important selenoamino acids [selenomethionine (SeMet), selenoethionine (SeEt) and selenocystine (SeCys)] a HPLC-hydraulic high pressure nebulization (HHPN)-AFS system was assembled. The separation was accomplished on a C18 column with the help of trifluoroacetic acid (TFA) and methanol in water as eluent. The effects of methanol and TFA concentrations on the retention time were investigated. The argon and hydrogen gas flow rate on the AFS signal intensity was optimized. The detection limit and the retention time for SeCys, SeMet and SeEt were 50, 42 and 71 ng ml-1 and 2.1, 3.9 and 6.9 min, respectively.

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