Determination of PS I oligomerisation in various cyanobacterial strains and mutants by non-invasive methods

T. Zakar, L. Kovacs, S. Vajravel, E. Herman, M. Kis, H. Laczko-Dobos, Z. Gombos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

PSI trimer to monomer ratio in intact cyanobacterial cells and isolated thylakoids was analysed by two noninvasive, in vivo methods; low-temperature fluorescence emission and circular dichroism spectroscopy. We measured fluorescence emission spectra of cells upon chlorophyll (Chl, 436 nm) excitation. All three species – Synechocystis sp. PCC 6803, Anabaena sp. PCC 7120, and Spirulina platensis – showed shifted Chl peak, indicating they have different spectral properties. CD spectroscopy revealed the highest intensity at 515 nm (PSI peak) in Spirulina platensis cells, which may originate from PSI multi-oligomerisation. The most sensitive response to heat treatment in this strain was the oligomerisation of PSI RCs. PSI dimers and tetramers in Anabaena cells showed smaller changes of the CD signal upon the heat treatment compared to that of Synechocystis WT. The lack of γ-linolenic acid affected the filament morphology by the loss of the spiral shape and the PSI monomerisation in Spirulina I22.

Original languageEnglish
Pages (from-to)294-299
Number of pages6
JournalPhotosynthetica
Volume56
Issue number1
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Spirulina
Anabaena
circular dichroism spectroscopy
Synechocystis
Spirulina platensis
mutants
Spectrum Analysis
Hot Temperature
Fluorescence
heat treatment
fluorescence
cells
Synechocystis sp. PCC 6803
Thylakoids
alpha-Linolenic Acid
Chlorophyll
Circular Dichroism
linolenic acid
methodology
thylakoids

Keywords

  • carotenoids
  • pigment–protein interactions
  • temperature stress
  • xanthophylls

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Determination of PS I oligomerisation in various cyanobacterial strains and mutants by non-invasive methods. / Zakar, T.; Kovacs, L.; Vajravel, S.; Herman, E.; Kis, M.; Laczko-Dobos, H.; Gombos, Z.

In: Photosynthetica, Vol. 56, No. 1, 01.03.2018, p. 294-299.

Research output: Contribution to journalArticle

Zakar, T. ; Kovacs, L. ; Vajravel, S. ; Herman, E. ; Kis, M. ; Laczko-Dobos, H. ; Gombos, Z. / Determination of PS I oligomerisation in various cyanobacterial strains and mutants by non-invasive methods. In: Photosynthetica. 2018 ; Vol. 56, No. 1. pp. 294-299.
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