Determination of pI by measuring the current in the mobilization step of high-performance capillary isoelectric focusing. Analysis of transferrin forms

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Abstract

In high-performance isoelectric focusing in capillaries, the focusing pattern of a sample is obtained during the mobilization step. A current parameter can be assigned to each peak in this pattern. Keeping the experimental conditions unchanged, these current parameters characterize the positions of the respective substances in the pH gradient and therefore they can be used for the determination of the isoelectric points of the substances.

Original languageEnglish
Pages (from-to)403-406
Number of pages4
JournalJournal of Chromatography A
Volume545
Issue number2
DOIs
Publication statusPublished - Jun 7 1991

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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