Determination of phenytoin in plasma by molecularly imprinted solid-phase extraction

Andrea Bereczki, Antal Tolokán, George Horvai, Viola Horváth, Francesca Lanza, Andrew John Hall, Börje Sellergren

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112 Citations (Scopus)


A molecularly imprinted polymer (MIP) using phenytoin as template and methacrylamide as the functional monomer was prepared. The selectivity was measured by comparing capacity factors of phenytoin and other structurally related compounds. The polymer was evaluated as a selective sorbent in molecularly imprinted solid-phase extraction (MISPE). Several washing solvents were tested to study their ability to disrupt the non-specific interactions occurring between the sample and the polymer matrix and the role of water in the recognition process was also investigated. It was shown that the key step of successful sample extraction is the right choice of the washing solvent. Plasma samples spiked with phenytoin were analyzed by the MISPE methodology developed in this work. Method validation (intra- and inter-day precision, recovery, specificity) was carried out. The calibration curve showed good linearity in the 2.5-40 μg/ml range corresponding to therapeutically relevant plasma levels. The intra- and inter-day precision values were below the 15% limit established for bioanalytical methods. The results showed that the method could be successfully applied for the determination of phenytoin in plasma samples.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - Sep 28 2001


  • Molecularly imprinted solid-phase extraction
  • Phenytoin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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