Determination of paromomycin residues in turkey tissues by liquid chromatography/mass spectrometry

K. Róna, G. Klausz, E. Keller, M. Szakay, P. Laczay, M. Shem-Tov, P. Székely-Körmöczy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A liquid chromatography-electrospray-mass spectrometry method has been developed and validated for the determination of paromomycin in turkey muscle, liver and kidney, using kanamycin as internal standard. The method consists of solid-phase extractions on mixed-mode columns. The chromatographic separation was carried out on a C18 column using binary gradient elution containing acetonitrile and 5 mM pentafluoropropionic-acid in water. The method was evaluated for specificity, linearity, recovery, accuracy, limit of detection, limit of quantification, intra- and inter-day repeatability, and stability. It was proven to be selective, linear, precise and accurate over the concentration range tested (0.5 × MRL-2 × MRL for each tissue) with correlation coefficients >0.990. The method was successfully used for the residue determination of PARO from edible tissues of turkeys.

Original languageEnglish
Pages (from-to)3792-3798
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number30
DOIs
Publication statusPublished - Nov 15 2009

Fingerprint

Paromomycin
Liquid chromatography
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Tissue
Kanamycin
Liver
Muscle
Recovery
Water
Solid Phase Extraction
Limit of Detection
Kidney
Muscles
acetonitrile
perfluoropropionic acid

Keywords

  • Liquid chromatography/mass spectrometry
  • Paromomycin
  • Solid-phase extraction
  • Turkey

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Determination of paromomycin residues in turkey tissues by liquid chromatography/mass spectrometry. / Róna, K.; Klausz, G.; Keller, E.; Szakay, M.; Laczay, P.; Shem-Tov, M.; Székely-Körmöczy, P.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 30, 15.11.2009, p. 3792-3798.

Research output: Contribution to journalArticle

Róna, K. ; Klausz, G. ; Keller, E. ; Szakay, M. ; Laczay, P. ; Shem-Tov, M. ; Székely-Körmöczy, P. / Determination of paromomycin residues in turkey tissues by liquid chromatography/mass spectrometry. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2009 ; Vol. 877, No. 30. pp. 3792-3798.
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