Determination of panomifene in human plasma by high-performance liquid chromatography

V. Erdélyi-Tóth, E. Pap, J. Kralovánszky, E. Bojti, I. Klebovich

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Abstract

An ion-pair HPLC method was developed for the determination of the antiestrogenic drug panomifene, (E)-1,2-diphenyl-1-{4-[2-(2-hydroxyethylamino)etboxylphenyl}-3,3,3-trifluoropropene, in human plasma. Tamoxifen, 20 ng in 1 ml of plasma, was used as an internal standard. The compounds were isolated from plasma by liquid-solid extraction. Fluorescence detection was achieved by on-line photochemical conversion of the compounds into highly fluorescent phenanthrene derivatives. The sensitivity of the method was 1 ng/ml. The within-day and between-day precision, linearity, extraction recovery and stability of panomifene in plasma and in deproteinized plasma were determined for validation of the method. The method is suitable for measuring plasma levels of panomifene and tamoxifen and for pharmacokinetic studies.

Original languageEnglish
Pages (from-to)419-425
Number of pages7
JournalJournal of Chromatography A
Volume668
Issue number2
DOIs
Publication statusPublished - May 13 1994

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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