Determination of nifedipine in human plasma by high performance liquid chromatography using column-switching technique

George Horvai, Andrea Hrabéczy-Páll, Viola Horváth, Imre Klebovich

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19 Citations (Scopus)

Abstract

A highly sensitive reversed-phase HPLC method has been developed for the determination of nifedipine in human plasma with electrochemical detection. A liquid-liquid extraction procedure is used in sample preparation with an average extraction recovery of 75%. Removing the highly lipophilic plasma components using a special column switching technique reduced the duration of the HPLC measurement from 30 to 9 min. The method is applicable for the pharmacokinetic characterization and bioavailability study of a sustained-release (retard) formulation of nifedipine and for human drug monitoring as it is indicated by the validation of the analysis method. The assay gave a linear response over the concentration range 2.5-50 ng/ml. All the validation parameters are within the internationally required limits.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalMikrochimica Acta
Volume113
Issue number3-6
DOIs
Publication statusPublished - May 1 1994

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Keywords

  • HPLC
  • column switching technique
  • electrochemical detection
  • nifedipine
  • validation

ASJC Scopus subject areas

  • Analytical Chemistry

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