Determination of mycotoxin production of fusarium species in genetically modified maize varieties by quantitative flow immunocytometry

Hajnalka Bánáti, Béla Darvas, Szilvia Fehér-Tóth, Árpád Czéh, A. Székács

Research output: Contribution to journalArticle

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Abstract

Levels of mycotoxins produced by Fusarium species in genetically modified (GM) and near-isogenic maize, were determined using multi-analyte, microbead-based flow immunocytometry with fluorescence detection, for the parallel quantitative determination of fumonisin B1, deoxynivalenol, zearalenone, T-2, ochratoxin A, and aflatoxin B1. Maize varieties included the genetic events MON 810 and DAS-59122-7, and their isogenic counterparts. Cobs were artificially infested by F. verticillioides and F. proliferatum conidia, and contained F. graminearum and F. sporotrichoides natural infestation. The production of fumonisin B1 and deoxynivalenol was substantially affected in GM maize lines: F. verticillioides, with the addition of F. graminearum and F. sporotrichoides, produced significantly lower levels of fumonisin B1 (~300 mg·kg−1) in DAS-59122-7 than in its isogenic line (~580 mg·kg−1), while F. proliferatum, in addition to F. graminearum and F. sporotrichoides, produced significantly higher levels of deoxynivalenol (~18 mg·kg−1) in MON 810 than in its isogenic line (~5 mg·kg−1). Fusarium verticillioides, with F. graminearum and F. sporotrichoides, produced lower amounts of deoxynivalenol and zearalenone than F. proliferatum, with F. graminearum and F. sporotrichoides. T-2 toxin production remained unchanged when considering the maize variety. The results demonstrate the utility of the Fungi-Plex™ quantitative flow immunocytometry method, applied for the high throughput parallel determination of the target mycotoxins.

Original languageEnglish
Article number70
JournalToxins
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 22 2017

Fingerprint

amsonic acid
Mycotoxins
Fusarium
Zea mays
Zearalenone
T-2 Toxin
Aflatoxin B1
Fungal Spores
Fungi
Microspheres
Fluorescence
Throughput
deoxynivalenol
fumonisin B1

Keywords

  • Aflatoxin B1
  • DAS-59122-7
  • Deoxynivalenol
  • Flow cytometry
  • Fumonisin B1
  • Immunoanalysis
  • Maize
  • MON 810
  • Ochratoxin A
  • T-2
  • Zearalenone

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Determination of mycotoxin production of fusarium species in genetically modified maize varieties by quantitative flow immunocytometry. / Bánáti, Hajnalka; Darvas, Béla; Fehér-Tóth, Szilvia; Czéh, Árpád; Székács, A.

In: Toxins, Vol. 9, No. 2, 70, 22.02.2017.

Research output: Contribution to journalArticle

Bánáti, Hajnalka ; Darvas, Béla ; Fehér-Tóth, Szilvia ; Czéh, Árpád ; Székács, A. / Determination of mycotoxin production of fusarium species in genetically modified maize varieties by quantitative flow immunocytometry. In: Toxins. 2017 ; Vol. 9, No. 2.
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