Determination of MTDQ in human plasma by high performance liquid chromatography

J. Fekete, E. Pungor, G. Nagy, E. Kocsi, P. Csokn, F. Ziegler

Research output: Contribution to journalArticle

Abstract

A rapid, sensitive and selective normal phase chromatographic determination method is described for the determination of MTDQ/SensoradR/in human plasma. The drug was extracted into n-hexane-isoamylalcohol mixture (95,5/o,5 v/v) using a Partisil 5 μm chromatographic column, with n-hexane-methylenechloride-dioxane mixture (60/40/0,5 v/v) as eluent. Separation and quantification of MTDQ have been achieved within 15 min. The detection limit of the compound was 1 ng at 254 nm detection wavelength. The recovery was 90,4% and the coefficient of variation was found to be less than 4,5% in the concentration range of 0,01-10 μg/ml MTDQ in plasma. No drug, drug metabolite or endogenous substance interference was observed. Data on steady-state plasma concentration during long-term admonistration of the drug are presented. The effective concentration of MTDQ in plasma proved to be 1–7 μg/ml. The reproducibility and precision of normal and reversed phase chromatography of the drug were compared.

Original languageEnglish
Pages (from-to)2019-2038
Number of pages20
JournalJournal of Liquid Chromatography
Volume11
Issue number9-10
DOIs
Publication statusPublished - Jul 1 1988

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Plasma (human)
High performance liquid chromatography
High Pressure Liquid Chromatography
Pharmaceutical Preparations
Plasmas
Reverse-Phase Chromatography
Metabolites
Chromatography
Limit of Detection
6,6'-methylene bis(2,2,4-trimethyl-1,2-dihydroquinoline)
Recovery
Wavelength

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Determination of MTDQ in human plasma by high performance liquid chromatography. / Fekete, J.; Pungor, E.; Nagy, G.; Kocsi, E.; Csokn, P.; Ziegler, F.

In: Journal of Liquid Chromatography, Vol. 11, No. 9-10, 01.07.1988, p. 2019-2038.

Research output: Contribution to journalArticle

Fekete, J. ; Pungor, E. ; Nagy, G. ; Kocsi, E. ; Csokn, P. ; Ziegler, F. / Determination of MTDQ in human plasma by high performance liquid chromatography. In: Journal of Liquid Chromatography. 1988 ; Vol. 11, No. 9-10. pp. 2019-2038.
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