Determination of L-Lactic Acid Content in Foods by Enzyme-Based Amperometric Bioreactor

Zsuzsanna Bori, Gábor Csiffáry, Diána Virág, Marianna Tóth-Markus, Attila Kiss, N. Adányi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A thermostable lactate oxidase (from Aerococcus viridans) based bioreactor was elaborated for L-lactate determination in dairy products. The enzyme was immobilised in a thin-layer enzyme cell connected into a flow injection system with an amperometric detector. The linear measuring range was 0.1-1mmol/L L-lactate (R 2=0.99; linear regression equation: y=81.2x+3.3). The detection limit was 0.05mmol/L. 40 Samples were analyzed per hour. Considering reproducibility: the signals measured were very stable for over 800 injections, after this period the activity of the cell decreased slowly. The method was used for the control of dairy products and for monitoring milk freshness.

Original languageEnglish
Pages (from-to)158-164
Number of pages7
JournalElectroanalysis
Volume24
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Dairy products
lactate 2-monooxygenase
Lactic acid
Bioreactors
Lactic Acid
Enzymes
Linear regression
Cells
Detectors
Monitoring
Milk
Oxidoreductases

Keywords

  • Aerococcus viridans
  • Biosensors
  • Enzymes
  • Lactate oxidase
  • Thin-layer enzyme cell

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Determination of L-Lactic Acid Content in Foods by Enzyme-Based Amperometric Bioreactor. / Bori, Zsuzsanna; Csiffáry, Gábor; Virág, Diána; Tóth-Markus, Marianna; Kiss, Attila; Adányi, N.

In: Electroanalysis, Vol. 24, No. 1, 01.2012, p. 158-164.

Research output: Contribution to journalArticle

Bori, Zsuzsanna ; Csiffáry, Gábor ; Virág, Diána ; Tóth-Markus, Marianna ; Kiss, Attila ; Adányi, N. / Determination of L-Lactic Acid Content in Foods by Enzyme-Based Amperometric Bioreactor. In: Electroanalysis. 2012 ; Vol. 24, No. 1. pp. 158-164.
@article{191320f61c3a4475a795c30fab7988d4,
title = "Determination of L-Lactic Acid Content in Foods by Enzyme-Based Amperometric Bioreactor",
abstract = "A thermostable lactate oxidase (from Aerococcus viridans) based bioreactor was elaborated for L-lactate determination in dairy products. The enzyme was immobilised in a thin-layer enzyme cell connected into a flow injection system with an amperometric detector. The linear measuring range was 0.1-1mmol/L L-lactate (R 2=0.99; linear regression equation: y=81.2x+3.3). The detection limit was 0.05mmol/L. 40 Samples were analyzed per hour. Considering reproducibility: the signals measured were very stable for over 800 injections, after this period the activity of the cell decreased slowly. The method was used for the control of dairy products and for monitoring milk freshness.",
keywords = "Aerococcus viridans, Biosensors, Enzymes, Lactate oxidase, Thin-layer enzyme cell",
author = "Zsuzsanna Bori and G{\'a}bor Csiff{\'a}ry and Di{\'a}na Vir{\'a}g and Marianna T{\'o}th-Markus and Attila Kiss and N. Ad{\'a}nyi",
year = "2012",
month = "1",
doi = "10.1002/el1/6/2012 7:49:56 AMan.201100409",
language = "English",
volume = "24",
pages = "158--164",
journal = "Electroanalysis",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
number = "1",

}

TY - JOUR

T1 - Determination of L-Lactic Acid Content in Foods by Enzyme-Based Amperometric Bioreactor

AU - Bori, Zsuzsanna

AU - Csiffáry, Gábor

AU - Virág, Diána

AU - Tóth-Markus, Marianna

AU - Kiss, Attila

AU - Adányi, N.

PY - 2012/1

Y1 - 2012/1

N2 - A thermostable lactate oxidase (from Aerococcus viridans) based bioreactor was elaborated for L-lactate determination in dairy products. The enzyme was immobilised in a thin-layer enzyme cell connected into a flow injection system with an amperometric detector. The linear measuring range was 0.1-1mmol/L L-lactate (R 2=0.99; linear regression equation: y=81.2x+3.3). The detection limit was 0.05mmol/L. 40 Samples were analyzed per hour. Considering reproducibility: the signals measured were very stable for over 800 injections, after this period the activity of the cell decreased slowly. The method was used for the control of dairy products and for monitoring milk freshness.

AB - A thermostable lactate oxidase (from Aerococcus viridans) based bioreactor was elaborated for L-lactate determination in dairy products. The enzyme was immobilised in a thin-layer enzyme cell connected into a flow injection system with an amperometric detector. The linear measuring range was 0.1-1mmol/L L-lactate (R 2=0.99; linear regression equation: y=81.2x+3.3). The detection limit was 0.05mmol/L. 40 Samples were analyzed per hour. Considering reproducibility: the signals measured were very stable for over 800 injections, after this period the activity of the cell decreased slowly. The method was used for the control of dairy products and for monitoring milk freshness.

KW - Aerococcus viridans

KW - Biosensors

KW - Enzymes

KW - Lactate oxidase

KW - Thin-layer enzyme cell

UR - http://www.scopus.com/inward/record.url?scp=84855416116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855416116&partnerID=8YFLogxK

U2 - 10.1002/el1/6/2012 7:49:56 AMan.201100409

DO - 10.1002/el1/6/2012 7:49:56 AMan.201100409

M3 - Article

AN - SCOPUS:84855416116

VL - 24

SP - 158

EP - 164

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 1

ER -