Determination of infarct size in Ex Vivo swine hearts by multidetector computed tomography using gadolinium as contrast medium

Akos Varga-Szemes, Balazs Ruzsics, Robert Kirschner, Satinder P. Singh, Pal Kiss, Brigitta C. Brott, Tamas Simor, Ada Elgavish, Gabriel A. Elgavish

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Abstract

Objective: To demonstrate the feasibility of using multidetector computed tomography with gadolinium contrast (Gd-MDCT) for the quantification of myocardial infarct (MI). Materials and Methods: MI was induced in male swine (n = 6). One week later, the animals received 0.2-mmol/kg gadopentetate dimeglumine and were sacrificed. On the excised hearts, Gd-MDCT with several tube voltages (80, 120, and 140 kV), late gadolinium enhancement MRI (LGE-MRI), and triphenyl-tetrazolium-chloride staining were then conducted. We used a 2-SD threshold for the CT images and several threshold limits (2, 3, 4, 5, 6 SD, and full width at half-maximum [FWHM]) for the LGE-MRI images to delineate the infarct area. Total infarct volume and infarct fraction of each heart were calculated. Results: MI size measured by MDCT at 140 kV showed good correlation with the reference triphenyl-tetrazolium-chloride value. Applying an 80-kV tube voltage, however, significantly underestimated MI size. In our study, the LGE-MRI method, using the 6-SD threshold, provided the most accurate determination of MI size. LGE-MRI, using the 2-and 3-SD threshold limits, significantly overestimated infarct size. Conclusions: The Gd-MDCT technique has been found suitable for the evaluation of MI in an ex vivo experimental setting. Gd-MDCT has the ability to detect MI even at low kV settings, but accuracy is limited by a high image noise because of reduced photon flux.

Original languageEnglish
Pages (from-to)277-283
Number of pages7
JournalInvestigative Radiology
Volume47
Issue number5
DOIs
Publication statusPublished - May 1 2012

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Keywords

  • gadolinium-based contrast medium
  • magnetic resonance imaging
  • multidetector computed tomography
  • myocardial infarct

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Varga-Szemes, A., Ruzsics, B., Kirschner, R., Singh, S. P., Kiss, P., Brott, B. C., Simor, T., Elgavish, A., & Elgavish, G. A. (2012). Determination of infarct size in Ex Vivo swine hearts by multidetector computed tomography using gadolinium as contrast medium. Investigative Radiology, 47(5), 277-283. https://doi.org/10.1097/RLI.0b013e31823fabc6