Determination of hydrophobicity parameters of antibiotics by reversed-phase chromatography. The effect of support

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Abstract

The hydrophobicity and specific hydrophilic surface area of 29 antibiotics were determined by reversed-phase thin-layer chromatography using impregnated silica and alumina supports and methanol:water mixtures as eluents. It was found that the supports retain their original retention characteristics even after impregnation influencing the reversed-phase retention of antibiotics. It was established that the hydrophobicity parameters are intercorrelated both on impregnated silica and alumina layers, indicating that antibiotics behave as a homologous series of solutes. However, the correlations were not strong enough to substitute the parameters with each other in quantitative structure-activity relationship studies. The specific hydrophobic surface areas determined on impregnated silica and alumina showed no significant correlation, proving the decisive role of support characteristics in the determination of this parameter.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalBiomedical Chromatography
Volume10
Issue number3
DOIs
Publication statusPublished - May 1 1996

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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