Determination of H+/K+-ATPase activity in human gastric biopsy specimens

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Abstract

The aim of our work was to develop a method to determine the the H+/K+-ATPase activity of human gastric biopsy samples. Our method is based on the phosphatase activity and the K+-induceable property of the enzyme. K+-induceable pNPPase activity was determined from homogenated corpus and antrum biopsy samples. H+/K+ase activity was calculated as the difference between the corpus and antrum K+-induceable pNPPase activities. Quality control measurements were done during 20 successive days from pooled homogenates. The total, between-day and between-run, within-day and within-run coefficients of variations were between 10 and 16%. The healthy mean and reference range of K+induceable pNPPase activity in the corpus was 95.8 (95% CI: 83.4-108.2 mU/mg protein); in the antrum it was 28.3 (21.6-35.0) mU/mg protein. The calculated H+/K+-ATPase activity was 67.2 (56.9-77.5) mU/mg protein. The measured activities were independent of the age and gender. Summarizing our results we have concluded, that our novel method might be a potential tool to gather data about the functional acid producing capability of human gastric mucosa.

Original languageEnglish
Pages (from-to)743-745
Number of pages3
JournalClinical Chemistry and Laboratory Medicine
Volume38
Issue number8
DOIs
Publication statusPublished - Jan 1 2000

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Keywords

  • Acid output
  • Gastric mucosa
  • H/K-ATPase enzyme
  • Parietal cell

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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