Determination of HIV-1 subtypes in Hungary by synthetic peptides representing the V3 loop of env

K. Nagy, Éva BarabÁs, Viktória VÁrkonyi, A. Horváth

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

For the determination of HIV-1 diversity and serotyping of HIV-1 subtypes, an enzyme immunoassay was developed based on synthetic peptides representing immunodominant epitopes of the V3 loop of HIV-1 subtypes A, B, C and E, respectively. Sera from 53 asymptomatic HIV-1 infected individuals were tested for their pattern of binding reactivity to the synthetic peptides. 45/52 (85%) of the sera reacted exclusively to V3 peptide representing HIV-1 B subtypes, 4/52 (7.6%) of the sera showed cross reacticity to A/B peptides and 1/52 (1.9%) of the sera reacted with both A and C peptides. No single reactivity with subtype A or E peptides have been observed. Results together with nucleotide sequence analysis of the V3 region of clinical isolates suggest that HIV-1 infection in Hungary has been induced predominantly by strains belonging to HIV-1 subtype B.

Original languageEnglish
Pages (from-to)268-271
Number of pages4
JournalPathology and Oncology Research
Volume2
Issue number4
DOIs
Publication statusPublished - Dec 1996

Fingerprint

Hungary
HIV-1
Peptides
Serum
Immunodominant Epitopes
Serotyping
C-Peptide
Immunoenzyme Techniques
HIV Infections
Sequence Analysis

Keywords

  • HIV-1
  • Hungary
  • subtypes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oncology
  • Cancer Research

Cite this

Determination of HIV-1 subtypes in Hungary by synthetic peptides representing the V3 loop of env. / Nagy, K.; BarabÁs, Éva; VÁrkonyi, Viktória; Horváth, A.

In: Pathology and Oncology Research, Vol. 2, No. 4, 12.1996, p. 268-271.

Research output: Contribution to journalArticle

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