Determination of helicobacter priori cagA, vacA genotypes with real-time PCR melting curve analysis

Agnes Ruzsovics, B. Molnár, Zsuzsa Unger, Z. Tulassay, L. Prónai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objectives: genotypes of Helicobacter priori are the focus of interest because they play a prominent role in mucosal injury. The purpose of this study was to determine cagA and vacA genotypes of H. priori using real-time polymerase chain reaction (PCR) method with a double strain DNA binding SYBR Green I.dye, and to compare this with those of two immunohistochemical methods. Methods: Forty-three paraffin-embedded biopsy tissue samples were examined by histology, epidermal growth factor receptor (EGFR), proliferating cell nuclear antigen (PCNA) immunohistochemistry and melting curve analysis of real-time PCR. Results: The presence of cagA gene was associated with a significantly higher frequency of gastritis (P = 0.003) than that of vacA gene with intestinal metaplasia (P=0.045). Significant difference was found between the presence of cagA gene and EGFR expression in intestinal metaplasia cases in comparison with cagA negative samples (P = 0.0418). Statistically significant difference was detected between increased cell proliferation and the presence of gastritis. Conclusions: This method seems to be suitable for H. pylori genotype determination. Sensitivity, speed and simplicity are key areas in the development of PCR assays for H. pylori. Results supported the notion that infection with cagA positive H. priori strain causes more augmentated cell proliferation in the stomach mucosa.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalJournal of Physiology Paris
Volume95
Issue number1-6
DOIs
Publication statusPublished - 2001

Fingerprint

Helicobacter
Freezing
Real-Time Polymerase Chain Reaction
Genotype
Pylorus
Metaplasia
Gastritis
Cell Proliferation
erbB-1 Genes
Proliferating Cell Nuclear Antigen
Epidermal Growth Factor Receptor
Paraffin
Genes
Stomach
Histology
Mucous Membrane
Coloring Agents
Immunohistochemistry
Biopsy
Polymerase Chain Reaction

Keywords

  • CagA
  • Helicobacter pylori
  • Immunohistochemistry
  • Real-time PCR
  • VacA genotypes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology (medical)

Cite this

Determination of helicobacter priori cagA, vacA genotypes with real-time PCR melting curve analysis. / Ruzsovics, Agnes; Molnár, B.; Unger, Zsuzsa; Tulassay, Z.; Prónai, L.

In: Journal of Physiology Paris, Vol. 95, No. 1-6, 2001, p. 369-377.

Research output: Contribution to journalArticle

@article{fe75097951634d558332883796a3db87,
title = "Determination of helicobacter priori cagA, vacA genotypes with real-time PCR melting curve analysis",
abstract = "Objectives: genotypes of Helicobacter priori are the focus of interest because they play a prominent role in mucosal injury. The purpose of this study was to determine cagA and vacA genotypes of H. priori using real-time polymerase chain reaction (PCR) method with a double strain DNA binding SYBR Green I.dye, and to compare this with those of two immunohistochemical methods. Methods: Forty-three paraffin-embedded biopsy tissue samples were examined by histology, epidermal growth factor receptor (EGFR), proliferating cell nuclear antigen (PCNA) immunohistochemistry and melting curve analysis of real-time PCR. Results: The presence of cagA gene was associated with a significantly higher frequency of gastritis (P = 0.003) than that of vacA gene with intestinal metaplasia (P=0.045). Significant difference was found between the presence of cagA gene and EGFR expression in intestinal metaplasia cases in comparison with cagA negative samples (P = 0.0418). Statistically significant difference was detected between increased cell proliferation and the presence of gastritis. Conclusions: This method seems to be suitable for H. pylori genotype determination. Sensitivity, speed and simplicity are key areas in the development of PCR assays for H. pylori. Results supported the notion that infection with cagA positive H. priori strain causes more augmentated cell proliferation in the stomach mucosa.",
keywords = "CagA, Helicobacter pylori, Immunohistochemistry, Real-time PCR, VacA genotypes",
author = "Agnes Ruzsovics and B. Moln{\'a}r and Zsuzsa Unger and Z. Tulassay and L. Pr{\'o}nai",
year = "2001",
doi = "10.1016/S0928-4257(01)00050-X",
language = "English",
volume = "95",
pages = "369--377",
journal = "Journal de Physiologie",
issn = "0928-4257",
publisher = "Elsevier Masson SAS",
number = "1-6",

}

TY - JOUR

T1 - Determination of helicobacter priori cagA, vacA genotypes with real-time PCR melting curve analysis

AU - Ruzsovics, Agnes

AU - Molnár, B.

AU - Unger, Zsuzsa

AU - Tulassay, Z.

AU - Prónai, L.

PY - 2001

Y1 - 2001

N2 - Objectives: genotypes of Helicobacter priori are the focus of interest because they play a prominent role in mucosal injury. The purpose of this study was to determine cagA and vacA genotypes of H. priori using real-time polymerase chain reaction (PCR) method with a double strain DNA binding SYBR Green I.dye, and to compare this with those of two immunohistochemical methods. Methods: Forty-three paraffin-embedded biopsy tissue samples were examined by histology, epidermal growth factor receptor (EGFR), proliferating cell nuclear antigen (PCNA) immunohistochemistry and melting curve analysis of real-time PCR. Results: The presence of cagA gene was associated with a significantly higher frequency of gastritis (P = 0.003) than that of vacA gene with intestinal metaplasia (P=0.045). Significant difference was found between the presence of cagA gene and EGFR expression in intestinal metaplasia cases in comparison with cagA negative samples (P = 0.0418). Statistically significant difference was detected between increased cell proliferation and the presence of gastritis. Conclusions: This method seems to be suitable for H. pylori genotype determination. Sensitivity, speed and simplicity are key areas in the development of PCR assays for H. pylori. Results supported the notion that infection with cagA positive H. priori strain causes more augmentated cell proliferation in the stomach mucosa.

AB - Objectives: genotypes of Helicobacter priori are the focus of interest because they play a prominent role in mucosal injury. The purpose of this study was to determine cagA and vacA genotypes of H. priori using real-time polymerase chain reaction (PCR) method with a double strain DNA binding SYBR Green I.dye, and to compare this with those of two immunohistochemical methods. Methods: Forty-three paraffin-embedded biopsy tissue samples were examined by histology, epidermal growth factor receptor (EGFR), proliferating cell nuclear antigen (PCNA) immunohistochemistry and melting curve analysis of real-time PCR. Results: The presence of cagA gene was associated with a significantly higher frequency of gastritis (P = 0.003) than that of vacA gene with intestinal metaplasia (P=0.045). Significant difference was found between the presence of cagA gene and EGFR expression in intestinal metaplasia cases in comparison with cagA negative samples (P = 0.0418). Statistically significant difference was detected between increased cell proliferation and the presence of gastritis. Conclusions: This method seems to be suitable for H. pylori genotype determination. Sensitivity, speed and simplicity are key areas in the development of PCR assays for H. pylori. Results supported the notion that infection with cagA positive H. priori strain causes more augmentated cell proliferation in the stomach mucosa.

KW - CagA

KW - Helicobacter pylori

KW - Immunohistochemistry

KW - Real-time PCR

KW - VacA genotypes

UR - http://www.scopus.com/inward/record.url?scp=0034740030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034740030&partnerID=8YFLogxK

U2 - 10.1016/S0928-4257(01)00050-X

DO - 10.1016/S0928-4257(01)00050-X

M3 - Article

C2 - 11595462

AN - SCOPUS:0034740030

VL - 95

SP - 369

EP - 377

JO - Journal de Physiologie

JF - Journal de Physiologie

SN - 0928-4257

IS - 1-6

ER -