Determination of glycoalcaloids in Hungarian potatoes by HPLC

R. Tömösközi-Farkas, H. Daood, Zs Polgár, Gy Hajós

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this work potato genotypes originated from an intensive resistance breeding programme that includes unique combination of resistance to several pathogens and consumer response were analysed for glycoalcaloid content by reversed phase high performance liquid chromatographic method. The glycoalcaloids were concentrated from potato samples by solid-phase extraction with a disposable C18 cartridge column. Nucleosil 100-5 C-18-AB column was used with 50% acetonitrile in phosphate buffer as the mobile phase. The method was validated according to calibration curve, precision and recovery tests, limit of detection and quantitation. The results indicated that as low as 0.27 μg mL-1 can be quantitatively determined by this method with 94-98% and 99% for recovery and precision, respectively. Hungarian potatoes were analysed by the adapted procedure for their glycoalcaloid content. The result showed that the tested Hungarian potato varieties contain glycoalcaloids between 0.09 and 15 mg 100 g-1. The content of glycoalcaloids in the new varieties of potato was lower than the limit of the Official Food Regulations.

Original languageEnglish
JournalChromatographia
Volume63
Issue numberSUPPL. 13
DOIs
Publication statusPublished - Jun 2006

Fingerprint

Solanum tuberosum
High Pressure Liquid Chromatography
Recovery
Pathogens
Buffers
Phosphates
Calibration
Liquids
Solid Phase Extraction
Breeding
Limit of Detection
Genotype
Food
acetonitrile

Keywords

  • Column liquid chromatography
  • Glycoalcaloids
  • Potatoes
  • Resistance breeding

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Determination of glycoalcaloids in Hungarian potatoes by HPLC. / Tömösközi-Farkas, R.; Daood, H.; Polgár, Zs; Hajós, Gy.

In: Chromatographia, Vol. 63, No. SUPPL. 13, 06.2006.

Research output: Contribution to journalArticle

Tömösközi-Farkas, R. ; Daood, H. ; Polgár, Zs ; Hajós, Gy. / Determination of glycoalcaloids in Hungarian potatoes by HPLC. In: Chromatographia. 2006 ; Vol. 63, No. SUPPL. 13.
@article{097f0d7691374e32878e040da8646933,
title = "Determination of glycoalcaloids in Hungarian potatoes by HPLC",
abstract = "In this work potato genotypes originated from an intensive resistance breeding programme that includes unique combination of resistance to several pathogens and consumer response were analysed for glycoalcaloid content by reversed phase high performance liquid chromatographic method. The glycoalcaloids were concentrated from potato samples by solid-phase extraction with a disposable C18 cartridge column. Nucleosil 100-5 C-18-AB column was used with 50{\%} acetonitrile in phosphate buffer as the mobile phase. The method was validated according to calibration curve, precision and recovery tests, limit of detection and quantitation. The results indicated that as low as 0.27 μg mL-1 can be quantitatively determined by this method with 94-98{\%} and 99{\%} for recovery and precision, respectively. Hungarian potatoes were analysed by the adapted procedure for their glycoalcaloid content. The result showed that the tested Hungarian potato varieties contain glycoalcaloids between 0.09 and 15 mg 100 g-1. The content of glycoalcaloids in the new varieties of potato was lower than the limit of the Official Food Regulations.",
keywords = "Column liquid chromatography, Glycoalcaloids, Potatoes, Resistance breeding",
author = "R. T{\"o}m{\"o}sk{\"o}zi-Farkas and H. Daood and Zs Polg{\'a}r and Gy Haj{\'o}s",
year = "2006",
month = "6",
doi = "10.1365/s10337-006-0763-4",
language = "English",
volume = "63",
journal = "Chromatographia",
issn = "0009-5893",
publisher = "Springer Vieweg",
number = "SUPPL. 13",

}

TY - JOUR

T1 - Determination of glycoalcaloids in Hungarian potatoes by HPLC

AU - Tömösközi-Farkas, R.

AU - Daood, H.

AU - Polgár, Zs

AU - Hajós, Gy

PY - 2006/6

Y1 - 2006/6

N2 - In this work potato genotypes originated from an intensive resistance breeding programme that includes unique combination of resistance to several pathogens and consumer response were analysed for glycoalcaloid content by reversed phase high performance liquid chromatographic method. The glycoalcaloids were concentrated from potato samples by solid-phase extraction with a disposable C18 cartridge column. Nucleosil 100-5 C-18-AB column was used with 50% acetonitrile in phosphate buffer as the mobile phase. The method was validated according to calibration curve, precision and recovery tests, limit of detection and quantitation. The results indicated that as low as 0.27 μg mL-1 can be quantitatively determined by this method with 94-98% and 99% for recovery and precision, respectively. Hungarian potatoes were analysed by the adapted procedure for their glycoalcaloid content. The result showed that the tested Hungarian potato varieties contain glycoalcaloids between 0.09 and 15 mg 100 g-1. The content of glycoalcaloids in the new varieties of potato was lower than the limit of the Official Food Regulations.

AB - In this work potato genotypes originated from an intensive resistance breeding programme that includes unique combination of resistance to several pathogens and consumer response were analysed for glycoalcaloid content by reversed phase high performance liquid chromatographic method. The glycoalcaloids were concentrated from potato samples by solid-phase extraction with a disposable C18 cartridge column. Nucleosil 100-5 C-18-AB column was used with 50% acetonitrile in phosphate buffer as the mobile phase. The method was validated according to calibration curve, precision and recovery tests, limit of detection and quantitation. The results indicated that as low as 0.27 μg mL-1 can be quantitatively determined by this method with 94-98% and 99% for recovery and precision, respectively. Hungarian potatoes were analysed by the adapted procedure for their glycoalcaloid content. The result showed that the tested Hungarian potato varieties contain glycoalcaloids between 0.09 and 15 mg 100 g-1. The content of glycoalcaloids in the new varieties of potato was lower than the limit of the Official Food Regulations.

KW - Column liquid chromatography

KW - Glycoalcaloids

KW - Potatoes

KW - Resistance breeding

UR - http://www.scopus.com/inward/record.url?scp=33745144289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745144289&partnerID=8YFLogxK

U2 - 10.1365/s10337-006-0763-4

DO - 10.1365/s10337-006-0763-4

M3 - Article

VL - 63

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

IS - SUPPL. 13

ER -