Determination of glutathione and glutathione disulfide in biological samples: An in-depth review

Research output: Contribution to journalReview article

173 Citations (Scopus)


Glutathione (GSH) is a thiol-containing tripeptide, which plays central roles in the defence against oxidative damage and in signaling pathways. Upon oxidation, GSH is transformed to glutathione disulfide (GSSG). The concentrations of GSH and GSSG and their molar ratio are indicators of cell functionality and oxidative stress. Assessment of redox homeostasis in various clinical states and medical applications for restoration of the glutathione status are of growing importance. This review is intended to provide a state-of-the-art overview of issues relating to sample pretreatment and choices for the separation and detection of GSH and GSSG. High-performance liquid chromatography, capillary electrophoresis and gas chromatography (as techniques with a separation step) with photometric, fluorimetric, electrochemical and mass spectrometric detection are discussed, stress being laid on novel approaches.

Original languageEnglish
Pages (from-to)3331-3346
Number of pages16
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Issue number28
Publication statusPublished - Oct 15 2009


  • Glutathione
  • Glutathione disulfide
  • Glutathione status
  • Oxidative stress
  • Redox homeostasis
  • Thiol status

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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