Determination of fulvic acids in water samples of Hungarian caverns

Eniko Tatár, Eszter Csintalan, Victor G. Mihucz, Károly Tompa, László Pöppl, Gyula Záray

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Water samples from the region of Béke cavern of Aggtelek and above the Józsefhegyi and Pálvölgyi caverns of Buda, Hungary were analyzed by various chromatographic techniques and fluorescence spectrometry. According to size-exclusion chromatographic (SEC) measurements, the water samples contained only low-molecular-weight components, fulvic acids, which were concentrated and separated from inorganic and non-acidic organic compounds in the water samples at pH 2 using an XAD-8 resin column. Elution from the column was performed with 0.1 M NaOH solution. UV absorbance was monitored at 254 nm and fractions were collected and subsequently separated by applying a size-exclusion high-performance liquid chromatographic (SE-HPLC) method. The mobile phase was ammonium acetate at a concentration of 50 mmol/dm3 and a flow rate of 0.5 cm3/min. The SE-HPLC measurements confirmed the presence of low-molecular-weight compounds in the samples analyzed. Quantitative determination of the fulvic acid content of the concentrated samples was carried out by fluorescence spectrometry. The excitation and emission wavelengths were 325 and 440 nm, respectively. The fulvic acid concentrations of the water samples examined ranged between 2 and 112 μg/cm3.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalMicrochemical Journal
Volume73
Issue number1-2
DOIs
Publication statusPublished - Oct 1 2002

Keywords

  • Cavern
  • Fluorescence spectrometry
  • Fulvic acid
  • Size exclusion chromatography
  • XAD-8 resin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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