Determination of ethyl carbamate in wine by high performance liquid chromatography

Zsolt Ajtony, N. Szoboszlai, László Bencs, Erna Viszket, V. Mihucz

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Kinetics of pre-column derivatization with 9-xanthydrol for the determination of ethyl carbamate (EC) in wine by a previous high performance liquid chromatographic method with fluorescence detection was studied and further developed. The life-time of the derivatized product and its excitation/absorption spectra were systematically investigated. Using low acidity (pH = 2.5 set by phosphate buffers) only 3% of 9-xanthyl ethyl carbamate (XEC) decomposes in ~48 h, allowing a prolonged storage time of the derivatized EC conferring more accurate determination for large sample batches. Detection limit of this method is 3 μg L-1, while its average recovery is 98.5 ± 4.9%. Calibration is linear up to 400 μg L-1. The EC content in 33 Hungarian wine samples ranges from 4.9 to 39.9 μg L -1 (average: 17.7 μg L-1, median: 16.7 μg L -1), while only three of them was slightly over 30 μg L -1 EC, it being the maximum allowed concentration in countries already having legislation.

Original languageEnglish
Pages (from-to)1301-1305
Number of pages5
JournalFood Chemistry
Volume141
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

ethyl carbamate
Wine
Urethane
High performance liquid chromatography
wines
high performance liquid chromatography
High Pressure Liquid Chromatography
derivatization
Legislation
Acidity
laws and regulations
Calibration
Limit of Detection
acidity
Absorption spectra
storage time
Buffers
detection limit
calibration
buffers

Keywords

  • 9-Xanthydrol
  • Alcoholic beverages
  • Ethyl carbamate
  • Fluorescence detection
  • HPLC
  • Wine

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Determination of ethyl carbamate in wine by high performance liquid chromatography. / Ajtony, Zsolt; Szoboszlai, N.; Bencs, László; Viszket, Erna; Mihucz, V.

In: Food Chemistry, Vol. 141, No. 2, 2013, p. 1301-1305.

Research output: Contribution to journalArticle

Ajtony, Zsolt ; Szoboszlai, N. ; Bencs, László ; Viszket, Erna ; Mihucz, V. / Determination of ethyl carbamate in wine by high performance liquid chromatography. In: Food Chemistry. 2013 ; Vol. 141, No. 2. pp. 1301-1305.
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