Determination of ethyl carbamate in pálinka spirits by liquid chromatography-electrospray tandem mass spectrometry after derivatization

Edit Deák, Attila Gyepes, E. Stefanovits-Banyai, M. Dernovics

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A robust and selective method for the determination of ethyl carbamate in double-distilled fruit brandies ("pálinka" spirits) by HPLC-ESI-MS/MS is described in the study. The approach is based on the combination of xanthydrol derivatization and the multiple reaction monitoring (MRM) of xanthyl-ethyl carbamate. Interestingly, this compound could only be ionized with adequate intensity through [M+Na]+ adduct formation, similarly to some of the carbamate derived pesticides, as investigated with the help of synthesizing the commercially unavailable standard. 20 traditional spirit samples originating from 14 fruit species were analyzed with the developed method, showing high selectivity and fruit-dependent sensitivity, thus requiring standard addition for quantification purposes. The concentration range of ethyl carbamate in the spirit samples was between the detection limit (0.003mg L-1) and 2.6mg L-1. The study indicated that besides the usually high ethyl carbamate containing, stone fruit derived spirits namely, plum (Prunus domestica L.) and sour cherry (Prunus cerasus L.), spirit samples of quince (Cydonia oblonga Mill.), a non-stone fruit species, showed relatively high ethyl carbamate levels close to or exceeding the actual European recommendation of 1mg L-1. As quince is not known to contain either high amounts of cyanogenic glucoside or N-carbamyl-amino acids, this phenomenon cannot be actually referred to genuine inner parameters of this fruit.

Original languageEnglish
Pages (from-to)2452-2455
Number of pages4
JournalFood Research International
Volume43
Issue number10
DOIs
Publication statusPublished - Dec 2010

Fingerprint

ethyl carbamate
Urethane
derivatization
Tandem Mass Spectrometry
Liquid Chromatography
liquid chromatography
Fruit
Rosaceae
fruits
quinces
Prunus cerasus
brandy
Cydonia oblonga
Prunus domestica
stone fruits
carbamates
plums
Carbamates
sampling
Glucosides

Keywords

  • [M+Na]+
  • Ethyl carbamate
  • Liquid chromatography
  • Mass spectrometry
  • Quince
  • Spirits
  • Xanthydrol

ASJC Scopus subject areas

  • Food Science

Cite this

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abstract = "A robust and selective method for the determination of ethyl carbamate in double-distilled fruit brandies ({"}p{\'a}linka{"} spirits) by HPLC-ESI-MS/MS is described in the study. The approach is based on the combination of xanthydrol derivatization and the multiple reaction monitoring (MRM) of xanthyl-ethyl carbamate. Interestingly, this compound could only be ionized with adequate intensity through [M+Na]+ adduct formation, similarly to some of the carbamate derived pesticides, as investigated with the help of synthesizing the commercially unavailable standard. 20 traditional spirit samples originating from 14 fruit species were analyzed with the developed method, showing high selectivity and fruit-dependent sensitivity, thus requiring standard addition for quantification purposes. The concentration range of ethyl carbamate in the spirit samples was between the detection limit (0.003mg L-1) and 2.6mg L-1. The study indicated that besides the usually high ethyl carbamate containing, stone fruit derived spirits namely, plum (Prunus domestica L.) and sour cherry (Prunus cerasus L.), spirit samples of quince (Cydonia oblonga Mill.), a non-stone fruit species, showed relatively high ethyl carbamate levels close to or exceeding the actual European recommendation of 1mg L-1. As quince is not known to contain either high amounts of cyanogenic glucoside or N-carbamyl-amino acids, this phenomenon cannot be actually referred to genuine inner parameters of this fruit.",
keywords = "[M+Na]+, Ethyl carbamate, Liquid chromatography, Mass spectrometry, Quince, Spirits, Xanthydrol",
author = "Edit De{\'a}k and Attila Gyepes and E. Stefanovits-Banyai and M. Dernovics",
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T1 - Determination of ethyl carbamate in pálinka spirits by liquid chromatography-electrospray tandem mass spectrometry after derivatization

AU - Deák, Edit

AU - Gyepes, Attila

AU - Stefanovits-Banyai, E.

AU - Dernovics, M.

PY - 2010/12

Y1 - 2010/12

N2 - A robust and selective method for the determination of ethyl carbamate in double-distilled fruit brandies ("pálinka" spirits) by HPLC-ESI-MS/MS is described in the study. The approach is based on the combination of xanthydrol derivatization and the multiple reaction monitoring (MRM) of xanthyl-ethyl carbamate. Interestingly, this compound could only be ionized with adequate intensity through [M+Na]+ adduct formation, similarly to some of the carbamate derived pesticides, as investigated with the help of synthesizing the commercially unavailable standard. 20 traditional spirit samples originating from 14 fruit species were analyzed with the developed method, showing high selectivity and fruit-dependent sensitivity, thus requiring standard addition for quantification purposes. The concentration range of ethyl carbamate in the spirit samples was between the detection limit (0.003mg L-1) and 2.6mg L-1. The study indicated that besides the usually high ethyl carbamate containing, stone fruit derived spirits namely, plum (Prunus domestica L.) and sour cherry (Prunus cerasus L.), spirit samples of quince (Cydonia oblonga Mill.), a non-stone fruit species, showed relatively high ethyl carbamate levels close to or exceeding the actual European recommendation of 1mg L-1. As quince is not known to contain either high amounts of cyanogenic glucoside or N-carbamyl-amino acids, this phenomenon cannot be actually referred to genuine inner parameters of this fruit.

AB - A robust and selective method for the determination of ethyl carbamate in double-distilled fruit brandies ("pálinka" spirits) by HPLC-ESI-MS/MS is described in the study. The approach is based on the combination of xanthydrol derivatization and the multiple reaction monitoring (MRM) of xanthyl-ethyl carbamate. Interestingly, this compound could only be ionized with adequate intensity through [M+Na]+ adduct formation, similarly to some of the carbamate derived pesticides, as investigated with the help of synthesizing the commercially unavailable standard. 20 traditional spirit samples originating from 14 fruit species were analyzed with the developed method, showing high selectivity and fruit-dependent sensitivity, thus requiring standard addition for quantification purposes. The concentration range of ethyl carbamate in the spirit samples was between the detection limit (0.003mg L-1) and 2.6mg L-1. The study indicated that besides the usually high ethyl carbamate containing, stone fruit derived spirits namely, plum (Prunus domestica L.) and sour cherry (Prunus cerasus L.), spirit samples of quince (Cydonia oblonga Mill.), a non-stone fruit species, showed relatively high ethyl carbamate levels close to or exceeding the actual European recommendation of 1mg L-1. As quince is not known to contain either high amounts of cyanogenic glucoside or N-carbamyl-amino acids, this phenomenon cannot be actually referred to genuine inner parameters of this fruit.

KW - [M+Na]+

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KW - Liquid chromatography

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KW - Quince

KW - Spirits

KW - Xanthydrol

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