Determination of enzyme activity in biological fluids by means of electrochemical oxidation of NADH at a modified glassy carbon electrode

L. Bartalits, G. Nagy, E. Pungor

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This amperometric technique for the determination of enzyme activity is based on detecting a decrease in the concentration of the NADH co-factor of the enzyme reaction. A glassy carbon electrode, modified by adsorption of Mg2+ and NADH, is used to measure the anodic peak current that corresponds to the oxidation of NADH. We found no significant difference between the enzyme activity of lactate dehydrogenase (E.C. 1.1.1.27) preparations as measured by the above amperometric technique and by a spectrophotometric method.

Original languageEnglish
Pages (from-to)1780-1783
Number of pages4
JournalClinical chemistry
Volume30
Issue number11
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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