Determination of endogenous formaldehyde in plants (fruits) bound to L-arginine and its relation to the folate cycle, photosynthesis and apoptosis

L. Trézl, L. Hullán, T. Szarvas, A. Csiba, B. Szende

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A very powerful nucleophilic reagent, hydralazine(1-hydrazino-phtalazine) proved to be suitable for determination of the endogenous formaldehyde level in biological samples. It was found that in different plants (vegetables, fruits, especially in red beet, cauliflower, kohlrabi, grapes) is a large amount of releasable endogenous formaldehyde (0.5-1.0 mM) bound to L-arginine mainly in the form of N(G)-trihydroxymethyl-L-arginine (TriHMA). N(G)-hydroxymethyl-L-arginines (HMA) were proved to transfer their hydroxymethyl groups to tetrahydrofolic acid producing N5,N10-methylene-tetrahydrofolate, the coenzyme of thymidylate synthase. HMA was found to inhibit the cell proliferation of HT-29 cell culture (human colon adenocarcinoma ATCC HT-B 38) causing apoptosis. Photosynthetic experiments produced confirmatory evidences that 14CH2O could be formed in photosynthesis already after 10 seconds of 14CO2 fixation in the seedlings of Zea mays L. (single cross) and the 14CH2O was immediately trapped by L-arginine mainly as TriHMA.

Original languageEnglish
Pages (from-to)253-263
Number of pages11
JournalActa Biologica Hungarica
Volume49
Issue number2-4
Publication statusPublished - 1998

Fingerprint

Photosynthesis
apoptosis
Fruits
formaldehyde
Folic Acid
folic acid
Formaldehyde
arginine
Arginine
Fruit
photosynthesis
fruit
Apoptosis
fruits
vegetable
fixation
seedling
acid
thymidylate synthase
kohlrabi

Keywords

  • Apoptosis
  • Endogenous formaldehyde
  • Folate cycle
  • L-arginine
  • Photosynthesis
  • Plants

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Determination of endogenous formaldehyde in plants (fruits) bound to L-arginine and its relation to the folate cycle, photosynthesis and apoptosis. / Trézl, L.; Hullán, L.; Szarvas, T.; Csiba, A.; Szende, B.

In: Acta Biologica Hungarica, Vol. 49, No. 2-4, 1998, p. 253-263.

Research output: Contribution to journalArticle

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