Determination of biogenic amines in leafy vegetables by amino acid analyser

Livia Simon-Sarkadi, Wilhelm H. Holzapfel

Research output: Contribution to journalArticle

42 Citations (Scopus)


An automated ion-exchange Chromatographic method is described for determination of biogenic amines in leafy vegetables using a cation-exchange resin column in potassium form and a three-buffer system. The following amines were separated and quantified for Chinese cabbage, endive, iceberg lettuce and radiocchio: putrescine, histamine, cadaverine, spermidine, agmatine, spermine, tyramine. Amines from vegetable samples were extracted with 10% trichloroacetic acid. The total concentration of these amines ranged from 14 to 20 μg/g fresh weight. Spermidine was the major polyamine detected, present at concentrations of 7-15 μg/g fresh weight.

Original languageEnglish
Pages (from-to)230-233
Number of pages4
JournalZeitschrift für Lebensmittel-Untersuchung und -Forschung
Issue number3
Publication statusPublished - Mar 1 1994

ASJC Scopus subject areas

  • Food Science

Fingerprint Dive into the research topics of 'Determination of biogenic amines in leafy vegetables by amino acid analyser'. Together they form a unique fingerprint.

  • Cite this