A novel technique involving the coupling of high-performance liquid chromatography with atomic fluorescence spectrometry using ultrasonic nebulization as an interface was assessed for the determination of As III, Asv, dimethylarsinic and monomethylarsonic acid. The effects of hydrogen and argon gas flow rates, nebulization temperature, loop volume, pump rate and phosphate buffer conditions on signal intensities were investigated. The detection limit for AsIII, Asv, DMAs and MMAs were 35, 50, 20 and 20 ng, respectively (for 250 mm3 volume injected). Linearity ranges were 250-2500 ng for all four arsenic species.
- Arsenic speciation
- Atomic fluorescence spectrometry
- High-performance liquid chromatography
- Ultrasonic nebulization
ASJC Scopus subject areas
- Analytical Chemistry