Determination of apovincaminic acid in human plasma by gas-liquid chromatography

Margarita Polgár, L. Vereczkey

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

High-resolution capillary gas-liquid chromatography (GLC) and highly sensitive and selective nitrogen-phosphorus flame ionization detection were applied for the detection of apovincaminic acid (AVA), the main metabolite of the cerebral vasodilator vinpocetine in human plasma. AVA was recovered from plasma by ion-pair extraction and determined by GLC as its methyl ester after derivatization with diazomethane. An acid-base washing technique was used in order to a minimum. The lowest detection limit of the method is 2 ng of AVA per ml of plasma.

Original languageEnglish
Pages (from-to)29-32
Number of pages4
JournalJournal of Chromatography A
Volume241
Issue number1
DOIs
Publication statusPublished - May 28 1982

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Plasma Gases
Liquid chromatography
Gas chromatography
Gas Chromatography
vinpocetine
Diazomethane
Plasma (human)
Plasmas
Flame Ionization
Metabolites
Vasodilator Agents
Washing
Phosphorus
Ionization
Limit of Detection
Esters
Nitrogen
Ions
Acids
apovincaminic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Determination of apovincaminic acid in human plasma by gas-liquid chromatography. / Polgár, Margarita; Vereczkey, L.

In: Journal of Chromatography A, Vol. 241, No. 1, 28.05.1982, p. 29-32.

Research output: Contribution to journalArticle

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