Determination of ambroxol in dog plasma by high-performance liquid chromatography and UV detection

B. Dalmadi Kiss, K. Balogh Nemes, I. Ürmös, J. Szúnyog, I. Klebovich

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A new sensitive HPLC-UV method has been developed and validated for the determination of ambroxol in dog plasma enabling the investigation of a newly developed 75 mg ambroxol-containing retard capsule of EGIS Pharmaceuticals Ltd., Budapest, Hungary. A gradient method was used for removing the longer retained plasma components of no interest. The separation was performed on a BDS Hypersil C18 (5 μm, 250 x 2.1 mm) analytical column, supplied with a 10 mm guard column containing the same packing material. The detection was performed at 210 nm. The calibration curve was linear in the range 25-2000 ng · mL-1. Nerisopam (EGIS-6775) was used as internal standard.

Original languageEnglish
Pages (from-to)S217-S220
JournalChromatographia
Volume51
Issue number1
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Ambroxol
  • Column liquid chromatography
  • Dog plasma
  • Solid phase extraction
  • UV detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Determination of ambroxol in dog plasma by high-performance liquid chromatography and UV detection'. Together they form a unique fingerprint.

  • Cite this