Determination of acyclovir in dog plasma by HPLC using a column switching technique

K. Balogh Nemes, B. Dalmadi Kiss, I. Klebovich

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new HPLC-UV method has been developed and validated for the pharmacokinetic linearity study of Telviran® tablets containing 200, 400, and 800 mg acyclovir. RP-18 solid phase extraction has been developed for sample preparation. Guanosine (9-[β-D-ribofuranosyl]guanine) was used as internal standard. The separation was carried out on an ODS Hypersil (5 μm, 200 x 4.5 mm) analytical column, supplied with a 20 mm guard column containing the same packing material. A column switching technique was applied for the elimination of the endogenous compounds eluting with longer retention times than the investigated compounds, so the analysis time was considerably shorter compared with the time of gradient elution. The eluent was 0.5% triethylamine in water, the pH was adjusted with orthophosphoric acid (85%) to pH5. The detection was performed at 254 nm. The calibration curve was linear in the concentration range 10-5000 ng mL-1. The new bioanalytical method was successfully applied for a pharmacokinetic linearity study in dogs.

Original languageEnglish
Pages (from-to)S211-S216
JournalChromatographia
Volume51
Issue numberSUPPL. 2
DOIs
Publication statusPublished - 2000

Keywords

  • Acyclovir
  • Column liquid chromatography
  • Column switching
  • Dog plasma
  • Solid phase extraction
  • UV detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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