Determination of active hydrogen in organic compounds by electron‐impact mass spectrometry

Zoltán Dinya, Miklós Lipták, Pál Szabó, Ferenc Sztaricskai, Karoly Vékey

Research output: Contribution to journalArticle

7 Citations (Scopus)


We report here detailed studies of Bose et al.'s (J. Org. Chem. 47, 4008 (1982)) simple method for obtaining electron‐impact (EI) mass spectra from non‐volatile and/or thermolabile organic compounds by using ammonium salts. Good quality mass spectra can be obtained by mixing the analyte with different ammonium salts (mainly with NH4Cl) in a given ratio, the mixture being introduced through a conventonal direct probe. A wide variety of ammonium salts has been used to study the effect of the anion and to determine the optimum analyte:NH4X ratio and the other experimental parameters (i.e., electron energy, source temperature etc). A very simple and cheap means of determination of active hydrogen in hydroxyl, carboxyl, sulfhydryl, amino and amido etc groups by EI mass spectrometry using deuterated ammonium chloride (ND4CI) is described. The method is based on exchange of active hydrogen for deuterium occurring in situ in the ion source. This technique allows drect comparison of spectra before and after deuteration and the number of active hydrogens can be identified in most cases. The applicability of this method is demonstrated on different model compounds.

Original languageEnglish
Pages (from-to)679-683
Number of pages5
JournalRapid Communications in Mass Spectrometry
Issue number11
Publication statusPublished - Nov 1992

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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