Determination of α-methyldopa in human plasma by validated high-performance liquid chromatography with fluorescence detection

K. Róna, K. Ary, B. Gachályi, I. Klebovich

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A sensitive reversed-phase gradient elution high-performance liquid chromatographic method with fluorescence detection has been developed for the determination of a-methyldopa (AMD) in human plasma. Separation of the investigated compound and the 3,4-dihydroxyphenylalanine (DOPA) internal standard was achieved on a Nucleosil 7 C18 column with a 5 mM heptanesulphonic acid sodium salt containing 0.05 M potassium dihydrogenphosphate (pH 3,2)-acetonitrile mobile phase. The composition of the mobile phase was changed according to a linear gradient time program. Detection was performed at 270 nm fluorimetric excitation and 320 nm emission. The compounds were isolated from plasma by Bond-Elut C18 solid-phase extraction. The limit of quantitation was found to be 10 ng/ml plasma. The assay was validated with respect to accuracy, precision and system suitability. All validated parameters were found to be within the 20% required limits. On the basis of the sensitivity, linearity and validation parameters the developed analytical method was found to be suitable for application in a bioequivalency study.

Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalJournal of Chromatography A
Volume730
Issue number1-2
DOIs
Publication statusPublished - Apr 12 1996

Keywords

  • DOPA
  • Methyldopa

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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