Determination and analysis of human serum alpha-1-acid glycoprotein by liquid chromatographic methods

T. Kremmer, M. Boldizsar, J. Kovacs, E. Paulik, K. Bencsik, B. Szajáni

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Human serum alpha-1-acid glycoprotein (orosomucoid, AGP) with particular interest as a tumor marker was measured, separated, purified and analyzed for its carbohydrate constituents. In order to demonstrate the changes in the concentration and composition of serum AGP induced by malignant diseases an improved method of sample preparation based on solvent extraction, ion exchange and reversed phase liquid chromatographic methods were applied. In preliminary studies significantly elevated serum AGP levels and increased fucose content in the carbohydrate moiety were found in cancer patients in comparison to the healthy individuals.

Original languageEnglish
Pages (from-to)1207-1218
Number of pages12
JournalJournal of Liquid Chromatography
Volume18
Issue number6
Publication statusPublished - 1995

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Orosomucoid
Carbohydrates
Fucose
Liquids
Solvent extraction
Tumor Biomarkers
Serum
Ion exchange
Ion Exchange
Chemical analysis
Neoplasms

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Determination and analysis of human serum alpha-1-acid glycoprotein by liquid chromatographic methods. / Kremmer, T.; Boldizsar, M.; Kovacs, J.; Paulik, E.; Bencsik, K.; Szajáni, B.

In: Journal of Liquid Chromatography, Vol. 18, No. 6, 1995, p. 1207-1218.

Research output: Contribution to journalArticle

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