Búza (Triticum aestivum L.)/Triticum timopheevii Zhuk. addíció, szubsztitúció és búza/rozs transzlokáció kimutatása C-sávozással és in situ hibridizációval

Translated title of the contribution: Detection of wheat (Triticum aestivum L.)/Triticum timopheevii Zhuk. addition and substitution and wheat/rye translocation by C-banding and in situ hybridization

Márta Lángné Molnár, Béla Koszegi, G. Linc, J. Sutka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Alien chromosomes and chromosome segments have been demonstrated in genetic material produced in the Department of Genetics of the Agricultural Research Institute, Martonvásár and in Martonvásár wheat varieties using two cytogenetical methods, i.e. C-banding and in situ hybridization. The advantage of C-banding is that individual chromosomes can be clearly identified by this method, but when applying in situ hybridization shorter chromosome segments built into an alien genome can be recognized, which do not carry typically stained bands. In addition, the translocation breakpoint in an alien translocation can be exactly determined using in situ hybridization. A 6G Triticum aestivum L./T. timopheevi Zhuk. addition line and a 6G(6B) T. timopheevii Zhuk.(T. aestivum L.) substitution line were identified in the progenies from T. aestivum L. x T. timopheevii Zhuk. crossing. The 44-chromosome 6G addition line is a BC1F1 progeny of the T. timopheevii Zhuk. x. T. aestivum L. cv. Mv 9 krl hybrid (Farshadfar et al. 1994) backcrossed with the wheat variety Mv 9. The 6G(6B) disomic substitution line was found among selected 42-chromosome progenies of a T. aestivum L. cv. Fleischmann 481 x T. timopheevii Zhuk. amphiploid (Belea 1961) backcrossed once with the wheat variety Mironovskaya 808 and three times with the wheat variety Mv 14 and subsequently self-pollinated. The 1B/1R wheat/rye translocation occurring in Martnonvásár wheat varieties was demonstrated by both C-banding and in situ hybridization. There was no difference in the size of the translocated rye chromosome arms among Martonvásár wheat varieties of different quality. The translocation breakpoint was at the centromere in each case.

Original languageHungarian
Pages (from-to)237-245
Number of pages9
JournalNovenytermeles
Volume45
Issue number3
Publication statusPublished - Jun 1996

Fingerprint

Triticum timopheevii
chromosome banding
rye
in situ hybridization
Triticum aestivum
wheat
chromosomes
substitution lines
chromosome addition
disomics
centromeres
agricultural research
genome

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Búza (Triticum aestivum L.)/Triticum timopheevii Zhuk. addíció, szubsztitúció és búza/rozs transzlokáció kimutatása C-sávozással és in situ hibridizációval. / Lángné Molnár, Márta; Koszegi, Béla; Linc, G.; Sutka, J.

In: Novenytermeles, Vol. 45, No. 3, 06.1996, p. 237-245.

Research output: Contribution to journalArticle

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