A citogenetikai vizsgálat és fluoreszcencia in situ hibridizáció párhuzamos alkalmazásának jelentósége krónikus granulocytás leukaemiában és myelodysplasiás szindrómában a 8-as triszómia és a 7-es monoszómia kimutatására.

Translated title of the contribution: Detection of trisomy 8 and monosomy 7 in chronic granulocytic leukemia and myelodysplastic syndrome by cytogenetic analysis and fluorescence in situ hybridization

P. Szabó Gabriella, Erzsébet Balogh, Zsuzsanna Jakab, Péter Germán, Ferencné Bodnár, Attila Kiss, Béla Telek, E. Oláh

Research output: Contribution to journalArticle

Abstract

INTRODUCTION AND AIMS: In this retrospective study the authors studied how interphase fluorescence in situ hybridization can be applied to the diagnosis of numerical chromosomal anomalies failed to be identified by cytogenetic analysis. METHODS: Thirty-four patients, 27 with chronic granulocytic leukemia and seven with myelodysplastic syndrome, were studied in order to identify disease-specific aberrations, trisomy 8 and monosomy 7 using both traditional cytogenetic analysis and fluorescence in situ hybridization on interphase bone marrow cells. Using alphoid-satellite centromeric specific probes, trisomy 8 indicating the progression of the disease and poor prognosis in chronic granulocytic leukemia as well as monosomy 7 in myelodysplastic syndrome were identified. RESULTS: In 21 of 34 cases both methods led to the same results. In 13 patients fluorescence in situ hybridization making possible to examine a great number of cells, provided more information about the gain and loss of chromosomes above and clarified uncertain cytogenetic results. CONCLUSIONS: In both hematological malignancies, studied by the authors, fluorescence in situ hybridization proved a useful and sensitive method to determine chromosomes unrecognized or not accurately identified by the traditional cytogenetic analysis and to define the ratio of pathological cells. At the same time the results confirm that conventional cytogenetic analysis is still essential in diagnosing the genetic alterations of malignant cells and point at the chromosomes that are worth further studying by other methods.

Original languageHungarian
Pages (from-to)2775-2779
Number of pages5
JournalOrvosi Hetilap
Volume143
Issue number50
Publication statusPublished - Dec 15 2002

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Cytogenetic Analysis
Myelodysplastic Syndromes
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Fluorescence In Situ Hybridization
Chromosomes
Interphase
Hematologic Neoplasms
Cytogenetics
Bone Marrow Cells
Disease Progression
Retrospective Studies
Cell Count
Monosomy Chromosome 7
Trisomy Chromosome 8

ASJC Scopus subject areas

  • Medicine(all)

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A citogenetikai vizsgálat és fluoreszcencia in situ hibridizáció párhuzamos alkalmazásának jelentósége krónikus granulocytás leukaemiában és myelodysplasiás szindrómában a 8-as triszómia és a 7-es monoszómia kimutatására. / Szabó Gabriella, P.; Balogh, Erzsébet; Jakab, Zsuzsanna; Germán, Péter; Bodnár, Ferencné; Kiss, Attila; Telek, Béla; Oláh, E.

In: Orvosi Hetilap, Vol. 143, No. 50, 15.12.2002, p. 2775-2779.

Research output: Contribution to journalArticle

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title = "A citogenetikai vizsg{\'a}lat {\'e}s fluoreszcencia in situ hibridiz{\'a}ci{\'o} p{\'a}rhuzamos alkalmaz{\'a}s{\'a}nak jelent{\'o}s{\'e}ge kr{\'o}nikus granulocyt{\'a}s leukaemi{\'a}ban {\'e}s myelodysplasi{\'a}s szindr{\'o}m{\'a}ban a 8-as trisz{\'o}mia {\'e}s a 7-es monosz{\'o}mia kimutat{\'a}s{\'a}ra.",
abstract = "INTRODUCTION AND AIMS: In this retrospective study the authors studied how interphase fluorescence in situ hybridization can be applied to the diagnosis of numerical chromosomal anomalies failed to be identified by cytogenetic analysis. METHODS: Thirty-four patients, 27 with chronic granulocytic leukemia and seven with myelodysplastic syndrome, were studied in order to identify disease-specific aberrations, trisomy 8 and monosomy 7 using both traditional cytogenetic analysis and fluorescence in situ hybridization on interphase bone marrow cells. Using alphoid-satellite centromeric specific probes, trisomy 8 indicating the progression of the disease and poor prognosis in chronic granulocytic leukemia as well as monosomy 7 in myelodysplastic syndrome were identified. RESULTS: In 21 of 34 cases both methods led to the same results. In 13 patients fluorescence in situ hybridization making possible to examine a great number of cells, provided more information about the gain and loss of chromosomes above and clarified uncertain cytogenetic results. CONCLUSIONS: In both hematological malignancies, studied by the authors, fluorescence in situ hybridization proved a useful and sensitive method to determine chromosomes unrecognized or not accurately identified by the traditional cytogenetic analysis and to define the ratio of pathological cells. At the same time the results confirm that conventional cytogenetic analysis is still essential in diagnosing the genetic alterations of malignant cells and point at the chromosomes that are worth further studying by other methods.",
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T1 - A citogenetikai vizsgálat és fluoreszcencia in situ hibridizáció párhuzamos alkalmazásának jelentósége krónikus granulocytás leukaemiában és myelodysplasiás szindrómában a 8-as triszómia és a 7-es monoszómia kimutatására.

AU - Szabó Gabriella, P.

AU - Balogh, Erzsébet

AU - Jakab, Zsuzsanna

AU - Germán, Péter

AU - Bodnár, Ferencné

AU - Kiss, Attila

AU - Telek, Béla

AU - Oláh, E.

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N2 - INTRODUCTION AND AIMS: In this retrospective study the authors studied how interphase fluorescence in situ hybridization can be applied to the diagnosis of numerical chromosomal anomalies failed to be identified by cytogenetic analysis. METHODS: Thirty-four patients, 27 with chronic granulocytic leukemia and seven with myelodysplastic syndrome, were studied in order to identify disease-specific aberrations, trisomy 8 and monosomy 7 using both traditional cytogenetic analysis and fluorescence in situ hybridization on interphase bone marrow cells. Using alphoid-satellite centromeric specific probes, trisomy 8 indicating the progression of the disease and poor prognosis in chronic granulocytic leukemia as well as monosomy 7 in myelodysplastic syndrome were identified. RESULTS: In 21 of 34 cases both methods led to the same results. In 13 patients fluorescence in situ hybridization making possible to examine a great number of cells, provided more information about the gain and loss of chromosomes above and clarified uncertain cytogenetic results. CONCLUSIONS: In both hematological malignancies, studied by the authors, fluorescence in situ hybridization proved a useful and sensitive method to determine chromosomes unrecognized or not accurately identified by the traditional cytogenetic analysis and to define the ratio of pathological cells. At the same time the results confirm that conventional cytogenetic analysis is still essential in diagnosing the genetic alterations of malignant cells and point at the chromosomes that are worth further studying by other methods.

AB - INTRODUCTION AND AIMS: In this retrospective study the authors studied how interphase fluorescence in situ hybridization can be applied to the diagnosis of numerical chromosomal anomalies failed to be identified by cytogenetic analysis. METHODS: Thirty-four patients, 27 with chronic granulocytic leukemia and seven with myelodysplastic syndrome, were studied in order to identify disease-specific aberrations, trisomy 8 and monosomy 7 using both traditional cytogenetic analysis and fluorescence in situ hybridization on interphase bone marrow cells. Using alphoid-satellite centromeric specific probes, trisomy 8 indicating the progression of the disease and poor prognosis in chronic granulocytic leukemia as well as monosomy 7 in myelodysplastic syndrome were identified. RESULTS: In 21 of 34 cases both methods led to the same results. In 13 patients fluorescence in situ hybridization making possible to examine a great number of cells, provided more information about the gain and loss of chromosomes above and clarified uncertain cytogenetic results. CONCLUSIONS: In both hematological malignancies, studied by the authors, fluorescence in situ hybridization proved a useful and sensitive method to determine chromosomes unrecognized or not accurately identified by the traditional cytogenetic analysis and to define the ratio of pathological cells. At the same time the results confirm that conventional cytogenetic analysis is still essential in diagnosing the genetic alterations of malignant cells and point at the chromosomes that are worth further studying by other methods.

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