Detection of transforming gene regions of human papillomavirus type 16 in cervical dysplasias by the polymerase chain reaction

J. Czeglédy, M. Evander, L. Veres, L. Gergely, G. Wadell

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Abstract

In a study of 29 cases of histologically confirmed, characterized colposcopically and cytologically, cervical intraepithelial neoplasias 48.3% (14/ 29) of biopsies were positive for human papillomavirus (HPV) type 16 DNA by polymerase chain reaction. We used two oligonucleotide primer pairs (position 215-514 and 606-805) flanking a 300 and a 199 base pair fragment from the early 6 (E6) and early 7 (E7) genes. The results were concordant both with the E6 and with the E7 regions. Of the amplified products 85.7% (12/14) could be confirmed; these carried 16 specific sequences by Southern blot hybridization. HPV 16 DNA was present in 6.7% (2/30) of the colposcopically directed cytologically normal matched control samples using the same methods.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalMedical Microbiology and Immunology
Volume180
Issue number1
DOIs
Publication statusPublished - Mar 1 1991

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

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