Infection caused by the parasite Toxoplasma gondii is often asymptomatic or it is a clinically mild disease. There are four groups in the population where this infection can cause serious problems. From these, three groups may be present at our department for diagnostic screening, namely pregnant women - who acquire the infection during the pregnancy, fetuses and newborns who are congenitally infected. Congenital toxoplasmosis is the result of a transplacental transmission of the Toxoplasma gondii from the recently infected mother. The risk of transmission depends on the gestational age, i.e. 7.2% between the 11th-14th gestational weeks and 67% between the 31st-34th gestational weeks. Toxoplasmosis leads to several fetal symptoms. Early diagnosis of the infection can enhance the success of medical treatment. Congenital toxoplasmosis can be detected by serological or amplification methods. The authors developed a fluorescent PCR and DNA fragment analysis technique for the detection of Toxoplasma gondii in the amniotic fluid. From the 64 samples studied, in 5 cases they were able to detect the genetic material of the protozoon. The introduced method is faster and more sensitive than the conventional PCR and could replace the ethidium bromide staining with an environment-friendly method.
|Translated title of the contribution||Detection of Toxoplasma gondii from amniotic fluid by fluorescent PCR and DNA fragment analysis|
|Number of pages||6|
|Journal||Magyar Noorvosok Lapja|
|Publication status||Published - Apr 19 2005|
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology