Detection of TNFα expression in the bone marrow and determination of TNFα production of peripheral blood mononuclear cells in myelodysplastic syndrome

Lenke Molnár, T. Berki, Alizadeh Hussain, P. Németh, Hajna Losonczy

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

TNFα is a highly active cytokine which plays an important role in the regulation of apoptotic cell death, a mechanism involved in the pathophysiology of myelodysplastic syndrome (MDS). In this study we investigated the expression of TNFα of the bone marrow trephine biopsies by immunohistochemical method and the TNFα production of peripheral blood mononuclear cells by ELISA method in 15 patients affected by MDS. Five of seven patients without excess of blasts showed high or intermediate TNFα expression in the bone marrow biopsies, whereas two patients with excess of blasts were negative and one had low expression. The five CMML patients revealed low or intermediate expression. The production of TNFα by the PBMC was analysed in 10 patients, four patients with RA and two with CMML produced higher level of TNFα which increased after stimulation with phorbol myristic acetate, but none of the RAEB patients revealed increase in TNFα production. In conclusion we suppose that increased TNFα expression and production by PBMC may be an indirect evidence of the role of increased apoptosis in low risk MDS patients.

Original languageEnglish
Pages (from-to)18-23
Number of pages6
JournalPathology and Oncology Research
Volume6
Issue number1
Publication statusPublished - 2000

Fingerprint

Myelodysplastic Syndromes
Blood Cells
Bone Marrow
Refractory Anemia with Excess of Blasts
Biopsy
Acetates
Cell Death
Enzyme-Linked Immunosorbent Assay
Apoptosis
Cytokines

Keywords

  • Apoptosis
  • MDS
  • Myelodysplastic syndrome
  • TNFα

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Detection of TNFα expression in the bone marrow and determination of TNFα production of peripheral blood mononuclear cells in myelodysplastic syndrome. / Molnár, Lenke; Berki, T.; Hussain, Alizadeh; Németh, P.; Losonczy, Hajna.

In: Pathology and Oncology Research, Vol. 6, No. 1, 2000, p. 18-23.

Research output: Contribution to journalArticle

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AU - Németh, P.

AU - Losonczy, Hajna

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AB - TNFα is a highly active cytokine which plays an important role in the regulation of apoptotic cell death, a mechanism involved in the pathophysiology of myelodysplastic syndrome (MDS). In this study we investigated the expression of TNFα of the bone marrow trephine biopsies by immunohistochemical method and the TNFα production of peripheral blood mononuclear cells by ELISA method in 15 patients affected by MDS. Five of seven patients without excess of blasts showed high or intermediate TNFα expression in the bone marrow biopsies, whereas two patients with excess of blasts were negative and one had low expression. The five CMML patients revealed low or intermediate expression. The production of TNFα by the PBMC was analysed in 10 patients, four patients with RA and two with CMML produced higher level of TNFα which increased after stimulation with phorbol myristic acetate, but none of the RAEB patients revealed increase in TNFα production. In conclusion we suppose that increased TNFα expression and production by PBMC may be an indirect evidence of the role of increased apoptosis in low risk MDS patients.

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