Detection of the multidrug resistant phenotype in human tumours by monoclonal antibodies and the streptavidin-biotinylated phycoerythrin complex method

Manfred Volm, Thomas Efferth, Mihaly Bak, Anthony D. Ho, Jürgen Mattern

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26 Citations (Scopus)

Abstract

The aim of this study was to find out whether the membrane glycoprotein P-170 can be detected in human tumours with both acquired and intrinsic resistance to chemotherapeutic agents using monoclonal antibodies ( 265 F4 and C219) and the streptavidin-biotinylated phycoerythrin complex method. Pretreated leukaemia cells and untreated lung and ovarian carcinomas were analysed. Two plasmacytomas and one leukaemia expressed high levels of P-glycoprotein, whereas two leukaemias showed moderate, and three leukaemias no expression of this protein. The intrinsic resistance was analysed with a panel of four human epidermoid lung cancer xenografts grown in nude mice. The expression of P-glycoprotein could be correlated with the degree of resistance. In addition, one out of five ovarian carcinomas revealed a high level of P-glycoprotein.

Original languageEnglish
Pages (from-to)743-749
Number of pages7
JournalEuropean Journal of Cancer and Clinical Oncology
Volume25
Issue number4
DOIs
Publication statusPublished - Apr 1989

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ASJC Scopus subject areas

  • Oncology

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