Az 1A/1R és az 1B/1R búza/rozs transzlokáció kimutatása az új martonvásári bú zafajtákban és fajtajelöltekben in situ hibridizá cióval

Translated title of the contribution: Detection of the 1A/1R and 1B/1R wheat/rye translocation in new Martonvásár wheat varieties and advanced lines using in situ hybridization

Éva Szakács, G. Linc, L. Láng, Márta Lángné Molnár

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Several types of rye (Secale cereale L.) translocation carrying major resistance genes are to be found in the foreign and Hungarian wheat varieties currently under cultivation. The 1B/1R translocation involving the 1RS chromosome arm from the rye variety Petkus can be detected in many of the Martonvásár wheat varieties registered in earlier years. The 1A/1R translocation was developed by irradiating octoploid triticale with X-rays, leading to the integration of the 1RS chromosome arm from the rye variety Insave F. A. into the wheat genome. The presence of this translocation was first detected by C-banding in the variety Mv Dalma. Biochemical markers and molecular cytogenetic methods are routinely used in Martonvásár to test advanced lines and newly registered varieties for the presence of rye translocations. In the present work, 13 new Martonvásár varieties and advanced lines were tested to discover whether they carried this rye chromosome segment. The presence of rye translocations was demonstrated using genomic in situ hybridization (GISH), while the chromosomes were identified by means of fluorescent in situ hybridization (FISH). The DNA probe used in FISH was the pSc119.2 repetitive sequence, which exhibits a specific hybridization pattern on the long arm of chromosomes 1A and 1B, allowing the two types of rye translocation to be identified unambiguously. It was demonstrated that, of the genotypes examined, Mv Táltos, Mv 07-03, Mv 08-03 and Mv 12-04 contained the 1A/1R translocation, while Mv Gorsium and Mv Waker carried the 1B/1R translocation. Pedigree analysis on the tested varieties indicated that the sources of the 1A/1R translocation in the Martonvásár wheat breeding programme were the wheat lines GA90078-I, F4549-W2-1 and F 4831, which can all be traced back to the wheat cultivar Amigo.

Original languageHungarian
Pages (from-to)527-534
Number of pages8
JournalNovenytermeles
Volume53
Issue number6
Publication statusPublished - 2004

Fingerprint

rye
in situ hybridization
wheat
chromosomes
fluorescence in situ hybridization
repetitive sequences
DNA probes
Secale cereale
triticale
chromosome banding
new variety
cytogenetics
pedigree
biomarkers
X-radiation
hybridization
genomics
genome
genotype
breeding

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Az 1A/1R és az 1B/1R búza/rozs transzlokáció kimutatása az új martonvásári bú zafajtákban és fajtajelöltekben in situ hibridizá cióval. / Szakács, Éva; Linc, G.; Láng, L.; Lángné Molnár, Márta.

In: Novenytermeles, Vol. 53, No. 6, 2004, p. 527-534.

Research output: Contribution to journalArticle

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title = "Az 1A/1R {\'e}s az 1B/1R b{\'u}za/rozs transzlok{\'a}ci{\'o} kimutat{\'a}sa az {\'u}j martonv{\'a}s{\'a}ri b{\'u} zafajt{\'a}kban {\'e}s fajtajel{\"o}ltekben in situ hibridiz{\'a} ci{\'o}val",
abstract = "Several types of rye (Secale cereale L.) translocation carrying major resistance genes are to be found in the foreign and Hungarian wheat varieties currently under cultivation. The 1B/1R translocation involving the 1RS chromosome arm from the rye variety Petkus can be detected in many of the Martonv{\'a}s{\'a}r wheat varieties registered in earlier years. The 1A/1R translocation was developed by irradiating octoploid triticale with X-rays, leading to the integration of the 1RS chromosome arm from the rye variety Insave F. A. into the wheat genome. The presence of this translocation was first detected by C-banding in the variety Mv Dalma. Biochemical markers and molecular cytogenetic methods are routinely used in Martonv{\'a}s{\'a}r to test advanced lines and newly registered varieties for the presence of rye translocations. In the present work, 13 new Martonv{\'a}s{\'a}r varieties and advanced lines were tested to discover whether they carried this rye chromosome segment. The presence of rye translocations was demonstrated using genomic in situ hybridization (GISH), while the chromosomes were identified by means of fluorescent in situ hybridization (FISH). The DNA probe used in FISH was the pSc119.2 repetitive sequence, which exhibits a specific hybridization pattern on the long arm of chromosomes 1A and 1B, allowing the two types of rye translocation to be identified unambiguously. It was demonstrated that, of the genotypes examined, Mv T{\'a}ltos, Mv 07-03, Mv 08-03 and Mv 12-04 contained the 1A/1R translocation, while Mv Gorsium and Mv Waker carried the 1B/1R translocation. Pedigree analysis on the tested varieties indicated that the sources of the 1A/1R translocation in the Martonv{\'a}s{\'a}r wheat breeding programme were the wheat lines GA90078-I, F4549-W2-1 and F 4831, which can all be traced back to the wheat cultivar Amigo.",
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AU - Linc, G.

AU - Láng, L.

AU - Lángné Molnár, Márta

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AB - Several types of rye (Secale cereale L.) translocation carrying major resistance genes are to be found in the foreign and Hungarian wheat varieties currently under cultivation. The 1B/1R translocation involving the 1RS chromosome arm from the rye variety Petkus can be detected in many of the Martonvásár wheat varieties registered in earlier years. The 1A/1R translocation was developed by irradiating octoploid triticale with X-rays, leading to the integration of the 1RS chromosome arm from the rye variety Insave F. A. into the wheat genome. The presence of this translocation was first detected by C-banding in the variety Mv Dalma. Biochemical markers and molecular cytogenetic methods are routinely used in Martonvásár to test advanced lines and newly registered varieties for the presence of rye translocations. In the present work, 13 new Martonvásár varieties and advanced lines were tested to discover whether they carried this rye chromosome segment. The presence of rye translocations was demonstrated using genomic in situ hybridization (GISH), while the chromosomes were identified by means of fluorescent in situ hybridization (FISH). The DNA probe used in FISH was the pSc119.2 repetitive sequence, which exhibits a specific hybridization pattern on the long arm of chromosomes 1A and 1B, allowing the two types of rye translocation to be identified unambiguously. It was demonstrated that, of the genotypes examined, Mv Táltos, Mv 07-03, Mv 08-03 and Mv 12-04 contained the 1A/1R translocation, while Mv Gorsium and Mv Waker carried the 1B/1R translocation. Pedigree analysis on the tested varieties indicated that the sources of the 1A/1R translocation in the Martonvásár wheat breeding programme were the wheat lines GA90078-I, F4549-W2-1 and F 4831, which can all be traced back to the wheat cultivar Amigo.

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KW - Genomic in situ hybridization

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